Efficient differentiation of human embryonic stem cells to definitive endoderm

被引：1284

作者：

D'Amour, KA ^{[1
]}

Agulnick, AD ^{[1
]}

Eliazer, S ^{[1
]}

Kelly, OG ^{[1
]}

Kroon, E ^{[1
]}

Baetge, EE ^{[1
]}

机构：

[1] CyThera Inc, San Diego, CA 92121 USA

来源：

NATURE BIOTECHNOLOGY | 2005年 / 23卷 / 12期

关键词：

D O I：

10.1038/nbt1163

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The potential of human embryonic stem (hES) cells to differentiate into cell types of a variety of organs has generated much excitement over the possible use of hES cells in therapeutic applications. Of great interest are organs derived from definitive endoderm, such as the pancreas. We have focused on directing hES cells to the definitive endoderm lineage as this step is a prerequisite for efficient differentiation to mature endoderm derivatives. Differentiation of hES cells in the presence of activin A and low serum produced cultures consisting of up to 80% definitive endoderm cells. This population was further enriched to near homogeneity using the cell-surface receptor CXCR4. The process of definitive endoderm formation in differentiating hES cell cultures includes an apparent epithelial-to-mesenchymal transition and a dynamic gene expression profile that are reminiscent of vertebrate gastrulation. These findings may facilitate the use of hES cells for therapeutic purposes and as in vitro models of development.

引用

页码：1534 / 1541

页数：8

共 64 条

[1] Feeder layer- and serum-free culture of human embryonic stem cells [J].