Use of RAPD markers to detect chimerism in synthetic grape chimeras (Vitis vinifera L.)

Shoot organogenesis was previously observed in vitro on a callus structure originating from an assemblage of two grape cultivars (Vitis vinifera L.): Chardonnay 7535 and Pinot noir 7613. Adventitious buds were assumed to be candidates of chimeras. RAPD analysis was used to distinguish between the two grapevine cultivars at a molecular level and to verify the hypothetical chimerical character of adventitious shoots.