Detection of genetically modified soybean in crude soybean oil

被引：17

作者：

Nikolic, Zorica ^{[1
]}

Vasiljevic, Ivana ^{[2
]}

Zdjelar, Gordana ^{[1
]}

Dordevic, Vuk ^{[1
]}

Ignjatov, Maja ^{[1
]}

Jovicic, Dusica ^{[1
]}

Milosevic, Dragana ^{[1
]}

机构：

[1] Inst Field & Vegetable Crops, Novi Sad, Serbia

[2] Bio Tech Lab, Vojvode Putnika Bb, Sremska Kamenic, Serbia

来源：

FOOD CHEMISTRY | 2014年 / 145卷

关键词：

GMO; Soybean; Crude oil; DNA; CHAIN;

D O I：

10.1016/j.foodchem.2013.09.017

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In order to detect presence and quantity of Roundup Ready (RR) soybean in crude oil extracted from soybean seed with a different percentage of GMO seed two extraction methods were used, CTAB and DNeasy Plant Mini Kit. The amplifications of lectin gene, used to check the presence of soybean DNA, were not achieved in all CTAB extracts of DNA, while commercial kit gave satisfactory results. Comparing actual and estimated GMO content between two extraction methods, root mean square deviation for kit is 0.208 and for CTAB is 2.127, clearly demonstrated superiority of kit over CTAB extraction. The results of quantification evidently showed that if the oil samples originate from soybean seed with varying percentage of RR, it is possible to monitor the GMO content at the first stage of processing crude oil. (C) 2013 Elsevier Ltd. All rights reserved.

引用

收藏

页码：1072 / 1075

页数：4

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