Free Radical Scavenging Activity of Kielmeyera variabilis (Clusiaceae)

被引:18
作者
Coqueiro, Aline [1 ]
Regasini, Luis Octavio [1 ]
Gutkoski Skrzek, Scheila Cristina [1 ]
Ferreira Queiroz, Marcos Marcal [1 ]
Siqueira Silva, Dulce Helena [1 ]
Bolzani, Vanderlan da Silva [1 ]
机构
[1] Sao Paulo State Univ, Inst Chem, Dept Organ Chem, BR-14800900 Araraquara, Brazil
基金
巴西圣保罗研究基金会;
关键词
Kielmeyera variabilis; Clusiaceae; antioxidant; free radical scavenging activity; DPPH center dot; ABTS(center dot+); flavonoid; flavonol; VITRO ANTIOXIDANT ACTIVITY; PTEROGYNE-NITENS; FLAVONOIDS; EXTRACTS; CONSTITUENTS; ABSORPTION; HEALTH; L;
D O I
10.3390/molecules18022376
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As part of our ongoing research on antioxidant agents from Brazilian flora, we screened the free radical scavenging activity of two extracts and eight fractions of Kielmeyera variabilis (Clusiaceae) using DPPH center dot (2,2-diphenyl-1-picrylhydrazyl-hydrate) and ABTS(center dot+) [2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid)] colorimetric assays. The ethyl acetate and n-butanol fractions of the leaves of K. variabilis displayed the strongest activity (IC50 of 3.5 +/- 0.3 and 4.4 +/- 0.2 mu g mL(-1) for DPPH center dot and 6.6 +/- 0.4 and 3.1 +/- 0.1 mu g mL(-1) for ABTS(center dot+), respectively). Chromatographic fractionation of the most potent fractions led to identification of three flavonols with previously described antioxidant activity, quercitrin (1), quercetin-3-O-beta-glucoside (3), and quercetin-3-O-beta-galactoside (4), and of one biflavone, podocarpusflavone A (2). This is the first time that the presence of these flavonoids in Kielmeyera variabilis has been reported.
引用
收藏
页码:2376 / 2385
页数:10
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