Anti-Oxidative and Anti-Aging Activities of Porcine By-Product Collagen Hydrolysates Produced by Commercial Proteases: Effect of Hydrolysis and Ultrafiltration

被引：55
作者：
Hong, Geun-Pyo [1 ]
Min, Sang-Gi [2 ]
Jo, Yeon-Ji [3 ]
机构：
[1] Sejong Univ, Dept Food Sci & Biotechnol, 209 Neungdong Ro, Seoul 05006, South Korea
[2] Konkuk Univ, Anim Resources Res Ctr, 120 Neungdong Ro, Seoul 05029, South Korea
[3] Univ Alberta, Dept Agr Food & Nutr Sci, 116 St & Ave, Edmonton, AB T6G 2P5, Canada
来源：
MOLECULES | 2019年 / 24卷 / 06期
基金：
新加坡国家研究基金会;
关键词：
hydrolysis; commercial proteases; collagen hydrolysate; antioxidant; anti-aging; FUNCTIONAL-PROPERTIES; ENZYMATIC-HYDROLYSIS; PROTEIN HYDROLYSATE; PEPTIDE FRACTIONS; PURIFICATION; IDENTIFICATION;
D O I：
10.3390/molecules24061104
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To investigate methods for improving the processing of porcine waste, porcine skin was hydrolyzed using different commercially available proteases (Alcalase, Flavorzyme, Neutrase, Bromeline, Protamex, and Papain) under several optimal conditions. Following enzymatic hydrolysis, the collagen hydrolysates (CHs) were fractionated by molecular weight (3 kDa) via membrane ultrafiltration. The CHs were analyzed for physical properties (pH, protein recovery, free amino group content, molecular weight distribution, and amino composition) as well as for functional properties (antioxidant activities and anti-aging activities). Among the CHs, CHs hydrolyzed by Alcalase (CH-Alcalase) exhibited the highest degree of hydrolysis compared to other CHs. Both CH-Alcalase and CH-Alcalase < 3 kDa fractions showed a considerably high antioxidant activity and collagenase inhibition activity. Therefore, resulting bioactives have potential for development as antioxidants and anti-aging ingredients in the food, cosmetics, and pharmaceuticals, from animal by-products.
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页数：14
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