Light-Responsive Helical Polypeptides Capable of Reducing Toxicity and Unpacking DNA: Toward Nonviral Gene Delivery

被引：148

作者：

Yin, Lichen ^{[1
]}

Tang, Haoyu ^{[1
]}

Kim, Kyung Hoon ^{[1
]}

Zheng, Nan ^{[1
]}

Song, Ziyuan ^{[1
]}

Gabrielson, Nathan P. ^{[1
]}

Lu, Hua ^{[1
]}

Cheng, Jianjun ^{[1
]}

机构：

[1] Univ Illinois, Dept Mat Sci & Engn, Urbana, IL 61801 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2013年 / 52卷 / 35期

基金：

美国国家科学基金会;

关键词：

cell-penetrating peptides; charge conversion; helical structures; nonviral gene delivery; TRANSFECTION EFFICIENCY; NUCLEAR-LOCALIZATION; SIDE-CHAINS; NANOPARTICLES; PEPTIDES; RELEASE; SIRNA; OPTIMIZATION; CYTOTOXICITY; COPOLYMERS;

D O I：

10.1002/anie.201302820

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Gene delivery vehicles: Helical, cationic polypeptides with light-responsive domains showed potent membrane activity and promoted efficient cellular internalization of DNA (see picture). After transfection, a light trigger induces protecting-group removal to expose anionic carboxylate groups. The reduced cationic charge and helix distortion of the polypeptides result in enhanced DNA unpacking and reduced material toxicity because of the eliminated membrane activity. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：9182 / 9186

页数：5

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