Structural studies of the O-specific polysaccharide(s) from the lipopolysaccharide of Azospirillum brasilense type strain Sp7

被引：23

作者：

Sigida, Elena N. ^{[1
]}

Fedonenko, Yuliya P. ^{[1
]}

Shashkov, Alexander S. ^{[2
]}

Zdorovenko, Evelina L. ^{[2
]}

Konnova, Svetlana A. ^{[1
,3
]}

Ignatov, Vladimir V. ^{[1
,3
]}

Knirel, Yuriy A. ^{[2
]}

机构：

[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410049, Russia

[2] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia

[3] Saratov NG Chernyshevskii State Univ, Saratov 410012, Russia

来源：

CARBOHYDRATE RESEARCH | 2013年 / 380卷

基金：

俄罗斯基础研究基金会;

关键词：

Lipopolysaccharide; Bacterial polysaccharide structure; Azospirillum brasilense; POLAR FLAGELLUM; WHEAT; MONOSACCHARIDES; CLASSIFICATION; RHIZOSPHERE; CHAIN; GENUS;

D O I：

10.1016/j.carres.2013.07.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipopolysaccharide was obtained by phenol-water extraction from dried bacterial cells of Azospirillum brasilense type strain Sp7. Mild acid hydrolysis of the lipopolysaccharide followed by GPC on Sephadex G-50 resulted in a polysaccharide mixture, which was studied by composition and methylation analyses, Smith degradation and H-1 and C-13 NMR spectroscopy. The following polysaccharide structures were established, where italics indicate a non-stoichiometric (similar to 40%) 2-O-methylation of L-rhamnose. [GRAPHICS] (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：76 / 80

页数：5

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