Biochemical and physiological responses in Caenorhabditis elegans exposed to sublethal concentrations of the organophosphorus insecticide, monocrotophos

被引：27

作者：

Leelaja, Bhadravathi Chandrappa ^{[1
]}

Rajini, Padmanabhan Sharda ^{[1
]}

机构：

[1] CSIR Cent Food Technol Res Inst, Food Protectants & Infestat Control Dept, Mysore 570020, Karnataka, India

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2013年 / 94卷

关键词：

Monocrotophos; Acetylcholinesterase; Carboxylesterase; Oxidative stress; Caenorhabditis elegans; OXIDATIVE STRESS; END-POINTS; CHOLINESTERASE ACTIVITY; LIFE-SPAN; NEMATODE; TOXICITY; CHLORPYRIFOS; PESTICIDE; CARBOXYLESTERASE; INHIBITION;

D O I：

10.1016/j.ecoenv.2013.04.015

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The objective of this study was to investigate the impact of sublethal concentrations of MCP on definitive markers of toxicity and oxidative balance in the nematode, Caenorhabditis elegans. Exposure of worms to 0.85, 1.7 and 3.4 mM (1/40, 1/20 and 1/10 LC50; LC50=34 mM) for 4 h at 20 degrees C induced significant perturbations in physiological parameters such as decreased brood size (47-73%), increased paralysis (47-85%) and inhibition of the activities of acetylcholinesterase (75-86%) and carboxylesterase (76-81%). These changes were accompanied by distinct oxidative impairments as evidenced by increased reactive oxygen species, decline in glutathione content and decrease in superoxide dismutase activity. Our results clearly demonstrate that low concentrations of MCP may alter the physiological and biochemical status in the nematode, thereby affecting the organism's fitness. Our findings on C elegans provide an easy diagnosis for OPI contamination and may become helpful in evaluating the ecotoxicological effects of OPI in the aquatic environment near agricultural fields. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：8 / 13

页数：6

共 71 条

[1]

Amanchi Nageswara Rao, 2011, Indian Journal of Fundamental and Applied Life Sciences, V1, P65

[2] Assessment of sublethal endpoints for toxicity testing with the nematode Caenorhabditis elegans [J].

Anderson, GL ;

Boyd, WA ;

Williams, PL .

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2001, 20 (04) :833-838

[3]

Bany IA, 2003, J NEUROSCI, V23, P8060

[4] Role of B-esterases in assessing toxicity of organophosphorus (chlorpyrifos, malathion) and carbamate (carbofuran) pesticides to Daphnia magna [J].

Barata, C ;

Solayan, A ;

Porte, C .

AQUATIC TOXICOLOGY, 2004, 66 (02) :125-139

[5]

BEERS RF, 1952, J BIOL CHEM, V195, P133

[6] COMPARATIVE TOXICITY, ANTICHOLINESTERASE ACTION AND METABOLISM OF METHYL PARATHION AND PARATHION IN SUNFISH AND MICE [J].

BENKE, GM ;

CHEEVER, KL ;

MIRER, FE ;

MURPHY, SD .

TOXICOLOGY AND APPLIED PHARMACOLOGY, 1974, 28 (01) :97-109

[7] Comparison of the sensitivity of three nematode species to copper and their utility in aquatic and soil toxicity tests [J].

Boyd, WA ;

Williams, PL .

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 2003, 22 (11) :2768-2774

[8]

BRENNER S, 1974, GENETICS, V77, P71

[9] Behavioral dysfunctions correlate to altered physiology in rainbow trout (Oncorynchus mykiss) exposed to cholinesterase-inhibiting chemicals [J].

Brewer S.K. ;

Little E.E. ;

DeLonay A.J. ;

Beauvais S.L. ;

Jones S.B. ;

Ellersieck M.R. .

Archives of Environmental Contamination and Toxicology, 2001, 40 (1) :70-76

[10] Serine hydrolase targets of organophosphorus toxicants [J].

Casida, JE ;

Quistad, GB .

CHEMICO-BIOLOGICAL INTERACTIONS, 2005, 157 :277-283

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