Role of 14-3-3-beta in the migration and invasion in human malignant glioma cell line U87MG

被引：14
作者：
Park, Sung-Geun [1 ]
Jung, Shin [1 ,2 ]
Ryu, Hyang-Hwa [2 ,3 ]
Jung, Tae-Young [1 ,2 ]
Moon, Kyung-Sub [1 ]
Kim, In-Young [1 ]
Jeong, Young-Il [2 ]
Pei, Jian [1 ,2 ]
Park, Seung-Jin [1 ]
Kang, Sam-Suk [1 ]
机构：
[1] Chonnam Natl Univ, Hwasun Hosp & Med Sch, Dept Neurosurg, Kwangju, South Korea
[2] Chonnam Natl Univ, Hwasun Hosp & Med Sch, Brain Tumor Res Lab, Res Inst Med Sci, Kwangju, South Korea
[3] Seonam Univ, Dept Clin Lab Sci, Namwon, South Korea
来源：
NEUROLOGICAL RESEARCH | 2012年 / 34卷 / 09期
基金：
新加坡国家研究基金会;
关键词：
Migration; Invasion; Glioma; 14-3-3-beta; YWHAB; AKT-PHOSPHORYLATION; 14-3-3-PROTEINS; PROTEINS; IDENTIFICATION; ASSOCIATION; ISOFORM; GROWTH; SURVIVAL; KINASES; BINDING;
D O I：
10.1179/1743132812Y.0000000087
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
Purpose: To assess the influence of 14-3-3-beta in modulating the migration and invasion of human glioma cells. Methods: To profile the genes associated with malignant glioma cell motility, differential display-polymerase chain reaction was performed and the findings were validated by Northern blotting in the U343MG-A, U87MG, and U87MG-10' human glioma cell lines. Antisense 14-3-3-beta cDNA plasmid was transfected into U87MG ('U87-YA-3'). To follow motility changes after transfection, simple scratch test and matrigel assay were performed. Morphological and cytoskeletal changes were documented by light and confocal microscopy. In addition, doubling times of the transfectant and endogenous 14-3-3-beta levels were determined in various glioma cell lines with different motilities. Results: 14-3-3-beta was highly expressed in U87MG cells. U87-YA-3 cells became small and flat, and actin was depolarized. Furthermore, U87-YA-3 cell motility was inhibited markedly versus parental U87MG cells. The doubling times of transfected and parent cells were 32 and 37 hours, respectively. Endogenous 14-3-3-beta expression in the human glioma cell lines was proportional to their migratory and invasive abilities. Conclusion: 14-3-3-beta modulates the migration and invasion in U87MG cells, which may be useful in developing therapeutic approaches for the treatment of glioma.
引用
收藏
页码：893 / 900
页数：8
相关论文
共 36 条
  • [1] Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants.
    Aitken, A
    [J]. PLANT MOLECULAR BIOLOGY, 2002, 50 (06) : 993 - 1010
  • [2] Baldin V, 2000, Prog Cell Cycle Res, V4, P49
  • [3] The IGF-I receptor in cell growth, transformation and apoptosis
    Baserga, R
    Hongo, A
    Rubini, M
    Prisco, M
    Valentinis, B
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 1997, 1332 (03): : F105 - F126
  • [4] Specific 14-3-3 isoform detection and immunolocalization in prion diseases
    Baxter, HC
    Fraser, JR
    Liu, WG
    Forster, JL
    Clokie, S
    Steinacker, P
    Otto, M
    Bahn, E
    Wiltfang, J
    Aitken, A
    [J]. BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 : 387 - 391
  • [5] INHIBITION OF PHOSPHATIDYLINOSITOL 3-KINASE ACTIVITY BY ASSOCIATION WITH 14-3-3-PROTEINS IN T-CELLS
    BONNEFOYBERARD, N
    LIU, YC
    VONWILLEBRAND, M
    SUNG, A
    ELLY, C
    MUSTELIN, T
    YOSHIDA, H
    ISHIZAKA, K
    ALTMAN, A
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) : 10142 - 10146
  • [6] Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor
    Brunet, A
    Bonni, A
    Zigmond, MJ
    Lin, MZ
    Juo, P
    Hu, LS
    Anderson, MJ
    Arden, KC
    Blenis, J
    Greenberg, ME
    [J]. CELL, 1999, 96 (06) : 857 - 868
  • [7] Prostate cancer metastasis: Talking the walk
    Coffey, DS
    [J]. NATURE MEDICINE, 1996, 2 (12) : 1305 - 1306
  • [8] 14-3-3-PROTEINS ASSOCIATE WITH CDC25-PHOSPHATASES
    CONKLIN, DS
    GALAKTIONOV, K
    BEACH, D
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) : 7892 - 7896
  • [9] 14-3-3 proteins: Structure, function, and regulation
    Fu, HA
    Subramanian, RR
    Masters, SC
    [J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 2000, 40 : 617 - 647
  • [10] 14-3-3 regulates actin dynamics by stabilizing phosphorylated cofilin
    Gohla, A
    Bokoch, GM
    [J]. CURRENT BIOLOGY, 2002, 12 (19) : 1704 - 1710
1234