An outbreak of classical swine fever in pigs in Bangladesh, 2015

被引:10
作者
Sarkar, Shamim [1 ]
Hossain, Mohammad Enayet [1 ]
Gurley, Emily S. [1 ]
Hasan, Rashedul [1 ]
Rahman, Mohammed Z. [1 ]
机构
[1] Icddr B, Div Infect Dis, Programme Emerging Infect, Dhaka, Bangladesh
关键词
Bangladesh; classical swine fever; outbreak; pig; PHYLOGENETIC ANALYSIS; ORAL IMMUNIZATION; WILD BOAR;
D O I
10.1002/vms3.81
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
In a group of 22 healthy pigs aged between 4 and 6 months, 2 pigs became ill with high fever, complete anorexia, cough and abnormal swaying movements on 22 June 2015. One of them died on June 24 and the second died on July 3. Shortly after, the remaining pigs also fell ill and died from the same illness by 10 August 2015. We investigated the aetiology, epidemiological and clinical features of the outbreak. We recorded the clinical signs and symptoms for each pig with the date of onset of illness. Veterinarians conducted post-mortem examinations on the 12 dead pigs, they collected tissue samples from the dead pigs and placed them in a tube containing 1mL of nucleic acid extraction buffer (lysis buffer). We tested all the tissue samples by real-time reverse transcription polymerase chain reaction (rRT-PCR) to detect classical swine fever virus (CSFV) because the animals' symptoms matched those of this disease. We also conducted a phylogentic analysis of the nucleotide sequence of the E2 gene segment of CSFV detected in a lung tissue sample. The attack rate (22/22) and the case fatality were 100%. The predominant symptoms of the disease included high fever, cough, diarrhoea and swaying movements of the hind legs prior to death. Of the 12 pigs tissue samples tested, all had evidence of the presence of CSFV RNA by rRT-PCR. The phylogenetic analysis indicated that the virus belongs to genotype 2.2, which is closely related to CSFV genotype 2.2 reported in India. Our investigation suggests that CSF is circulating in pigs, posing a risk for communities in Bangladesh that rely on pigs for economic income and dietary protein. Future research could focus on estimating the disease and economic burden of CSFV in pig rearing areas to determine if interventions might be warranted or cost-effective.
引用
收藏
页码:45 / 52
页数:8
相关论文
共 32 条
[1]   Classical Swine Fever-An Updated Review [J].
Blome, Sandra ;
Staubach, Christoph ;
Henke, Julia ;
Carlson, Jolene ;
Beer, Martin .
VIRUSES-BASEL, 2017, 9 (04) :1-24
[2]   Local spread of classical swine fever upon virus introduction into The Netherlands: Mapping of areas at high risk [J].
Boender, Gert Jan ;
Nodelijk, Gonnie ;
Hagenaars, Thomas J. ;
Elbers, Armin R. W. ;
de Jong, Mart C. M. .
BMC VETERINARY RESEARCH, 2008, 4 (1)
[3]   5′-UTR-based phylogenetic analysis of Classical swine fever virus isolates from India [J].
Desai, G. S. ;
Sharma, Anurag ;
Kataria, R. S. ;
Barman, N. N. ;
Tiwari, A. K. .
ACTA VIROLOGICA, 2010, 54 (01) :79-82
[4]   An experimental infection with classical swine fever in E2 subunit marker-vaccine vaccinated and in non-vaccinated pigs [J].
Dewulf, J ;
Laevens, H ;
Koenen, F ;
Vanderhallen, H ;
Mintiens, K ;
Deluyker, H ;
de Kruif, A .
VACCINE, 2000, 19 (4-5) :475-482
[5]  
Dias N.L., 2014, VET MED INT, V17, P4
[6]   The seroprevalence of foot-and-mouth disease in the sedentary livestock herds in four districts of Bhutan [J].
Dukpa, Kinzang ;
Robertson, Ian. D. ;
Ellis, Trevor. M. .
PREVENTIVE VETERINARY MEDICINE, 2011, 100 (3-4) :231-236
[7]   Classical swine fever:: the global situation [J].
Edwards, S ;
Fukusho, A ;
Lefèvre, PC ;
Lipowski, A ;
Pejsak, Z ;
Roehe, P ;
Westergaard, J .
VETERINARY MICROBIOLOGY, 2000, 73 (2-3) :103-119
[8]   REPRODUCTION OF PIGS IN THE HILLS OF EASTERN NEPAL [J].
GATENBY, RM ;
CHEMJONG, PB .
TROPICAL ANIMAL HEALTH AND PRODUCTION, 1992, 24 (03) :135-142
[9]  
Greiser-Wilke Irene, 2004, Animal Health Research Reviews, V5, P223, DOI 10.1079/AHR200472
[10]   Classical swine fever in Nepal [J].
Jha, Vijay Chandra ;
Karna, Vinay Kumar ;
Singh, Dinesh Kumar .
VETERINARY RECORD, 2012, 171 (01) :24-24