Type I J-Domain NbMIP1 Proteins Are Required for Both Tobacco Mosaic Virus Infection and Plant Innate Immunity

被引：44

作者：

Du, Yumei ^{[1
]}

Zhao, Jinping ^{[1
]}

Chen, Tianyuan ^{[1
]}

Liu, Qi ^{[1
]}

Zhang, Haili ^{[1
]}

Wang, Yan ^{[1
]}

Hong, Yiguo ^{[2
]}

Xiao, Fangming ^{[3
]}

Zhang, Ling ^{[4
]}

Shen, Qianhua ^{[4
]}

Liu, Yule ^{[1
]}

机构：

[1] Tsinghua Univ, Sch Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China

[2] Hangzhou Normal Univ, Sch Life & Environm Sci, Res Ctr Plant RNA Signaling, Hangzhou, Zhejiang, Peoples R China

[3] Univ Idaho, Dept Plant Soil & Entomol Sci, Moscow, ID 83843 USA

[4] Chinese Acad Sci, Inst Genet & Dev Biol, Beijing, Peoples R China

来源：

PLOS PATHOGENS | 2013年 / 9卷 / 10期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

RESISTANCE GENE TM-2(2); PROGRAMMED CELL-DEATH; ESCHERICHIA-COLI DNAJ; HEAT-SHOCK PROTEINS; DISEASE RESISTANCE; MOVEMENT PROTEIN; MOLECULAR CHAPERONE; MEDIATED RESISTANCE; FUNCTIONAL-ANALYSIS; HYPERSENSITIVE RESPONSE;

D O I：

10.1371/journal.ppat.1003659

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Tm-2(2) is a coiled coil-nucleotide binding-leucine rich repeat resistance protein that confers durable extreme resistance against Tomato mosaic virus (ToMV) and Tobacco mosaic virus (TMV) by recognizing the viral movement protein (MP). Here we report that the Nicotiana benthamiana J-domain MIP1 proteins (NbMIP1s) associate with tobamovirus MP, Tm-2(2) and SGT1. Silencing of NbMIP1s reduced TMV movement and compromised Tm-2(2)-mediated resistance against TMV and ToMV. Furthermore, silencing of NbMIP1s reduced the steady-state protein levels of ToMV MP and Tm-2(2). Moreover, NbMIP1s are required for plant resistance induced by other R genes and the nonhost pathogen Pseudomonas syringae pv. tomato (Pst) DC3000. In addition, we found that SGT1 associates with Tm-2(2) and is required for Tm-2(2)-mediated resistance against TMV. These results suggest that NbMIP1s function as co-chaperones during virus infection and plant immunity.

引用

页数：17

共 83 条

[1] Role of SGT1 in resistance protein accumulation in plant immunity [J].

Azevedo, Cristina ;

Betsuyaku, Shigeyuki ;

Peart, Jack ;

Takahashi, Akira ;

Noel, Laurent ;

Sadanandom, Ari ;

Casais, Catarina ;

Parker, Jane ;

Shirasu, Ken .

EMBO JOURNAL, 2006, 25 (09) :2007-2016

[2] Structure-Function Analysis of Barley NLR Immune Receptor MLA10 Reveals Its Cell Compartment Specific Activity in Cell Death and Disease Resistance [J].

Bai, Shiwei ;

Liu, Jie ;

Chang, Cheng ;

Zhang, Ling ;

Maekawa, Takaki ;

Wang, Qiuyun ;

Xiao, Wenkai ;

Liu, Yule ;

Chai, Jijie ;

Takken, Frank L. W. ;

Schulze-Lefert, Paul ;

Shen, Qian-Hua .

PLOS PATHOGENS, 2012, 8 (06)

[3] The Rx gene from potato controls separate virus resistance and cell death responses [J].

Bendahmane, A ;

Kanyuka, K ;

Baulcombe, DC .

PLANT CELL, 1999, 11 (05) :781-791

[4] RAR1 positively controls steady state levels of barley MLA resistance proteins and enables sufficient MLA6 accumulation for effective resistance [J].

Bieri, S ;

Mauch, S ;

Shen, QH ;

Peart, J ;

Devoto, A ;

Casais, C ;

Ceron, F ;

Schulze, S ;

Steinbiss, HH ;

Shirasu, K ;

Schulze-Lefert, P .

PLANT CELL, 2004, 16 (12) :3480-3495

[5] Structural and functional analysis of SGT1 reveals that its interaction with HSP90 is required for the accumulation of Rx, an R protein involved in plant immunity [J].

Boter, Marta ;

Amigues, Beatrice ;

Peart, Jack ;

Breuer, Christian ;

Kadota, Yasuhiro ;

Casais, Catarina ;

Moore, Geoffrey ;

Kleanthous, Colin ;

Ochsenbein, Francoise ;

Shirasu, Ken ;

Guerois, Raphael .

PLANT CELL, 2007, 19 (11) :3791-3804

[6]

Chapman SN, 1998, METH MOL B, V81, P123

[7]

Cheetham ME, 1998, CELL STRESS CHAPERON, V3, P28, DOI 10.1379/1466-1268(1998)003<0028:SFAEOD>2.3.CO

[8]

[9] Firefly luciferase complementation imaging assay for protein-protein interactions in plants [J].

Chen, Huamin ;

Zou, Yan ;

Shang, Yulei ;

Lin, Huiqiong ;

Wang, Yujing ;

Cai, Run ;

Tang, Xiaoyan ;

Zhou, Jian-Min .

PLANT PHYSIOLOGY, 2008, 146 (02) :368-376

[10] Effects of calreticulin on viral cell-to-cell movement [J].

Chen, MH ;

Tian, GW ;

Gafni, Y ;

Citovsky, V .

PLANT PHYSIOLOGY, 2005, 138 (04) :1866-1876

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