FISH probes for mouse chromosome identification

被引：48

作者：

Shi, YP

Mohapatra, G

Miller, J

Hanahan, D

Lander, E

Gold, P

Pinkel, D

Gray, J

机构：

[1] UNIV CALIF SAN FRANCISCO,CTR CANC,N415,SAN FRANCISCO,CA 94143

[2] UNIV CALIF SAN FRANCISCO,HORMONE RES INST,SAN FRANCISCO,CA 94143

[3] GENOME SYST INC,ST LOUIS,MO 63114

[4] WHITEHEAD INST BIOMED RES,CAMBRIDGE,MA 02142

[5] EO LAWRENCE BERKELEY LAB,BERKELEY,CA 94720

来源：

GENOMICS | 1997年 / 45卷 / 01期

关键词：

D O I：

10.1006/geno.1997.4904

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

P1 clones near the telomeres and centromeres of each mouse chromosome except Y have been selected from a mouse genomic library and mapped using fluorescence in situ hybridization (FISH). Each clone was selected to contain a genetically mapped polymorphic DNA sequence as close as possible to the centromere or telomere of a chromosome. The genetic distance from the various P1 clones to the most distal genetically mapped polymorphic sequence ranged from 0 for about half of the clones to 6.7 cM for the probe at the telomere of chromosome 14. The average distance to the most distal or proximal chromosome marker was 1.5 cM. The use of FISH with these probes for mouse chromosome identification during comparative genomic hybridization is illustrated. (C) 1997 Academic Press.

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页码：42 / 47

页数：6

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