A method for quick measurement of protein binding to unilamellar vesicles

被引：2
作者：
Zhan, HJ [1 ]
机构：
[1] Axys Pharmaceut Inc, S San Francisco, CA 94080 USA
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1999年 / 41卷 / 01期
关键词：
biotinylated lipid; liposome-binding; magnetic separation; human factor X;
D O I：
10.1016/S0165-022X(99)00030-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A general method for measuring interaction of Liposome-protein (or potentially small molecules) was developed. This method utilizes biotinylated liposomes to incubate with interactants. Streptavidin-coated paramagnetic resins were then added and the liposomes (along with bound materials) can be quickly separated under a magnetic field or by low speed centrifugation. Subsequently, concentration of unbound materials tin the supernatants) can be directly determined. The described method is particularly useful for proteins or compounds that are not very soluble under certain assay conditions. (C) 1999 Elsevier Science BN. All rights reserved.
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收藏
页码：13 / 19
页数：7
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