Exposure to the antifouling tributyltin (TBT) has been related to imposex in mollusks and to obesogenicity, adipogenesis and masculinization in fish. To understand the underlying molecular mechanisms, we evaluated dose-response effects of TBT (1.7-56 nM) in zebrafish eleutheroembryos transcriptome exposed from 2 to 5 days post-fertilization. RNA-sequencing analysis identified 3238 differentially expressed transcripts in eleutheroembryos exposed to TBT. Benchmark dose analyses (BMD) showed that the point of departure (PoD) for transcriptomic effects (9.28 nM) was similar to the metabolomic PoD (11.5 nM) and about one order of magnitude lower than the morphometric PoD (67.9 nM) or the median lethal concentration (LC50: 93.6 nM). Functional analysis of BMD transcriptomic data identified steroid metabolism and cholesterol and vitamin D-3 biosynthesis as the most sensitive pathways to TBT (< 50% PoD). Conversely, transcripts related to general stress and DNA damage became affected only at doses above the PoD. Therefore, our results indicate that transcriptomes can act as early molecular indicators of pollutant exposure, and illustrates their usefulness for the mechanistic identification of the initial toxic events. As the estimated molecular PoDs are close to environmental levels, we concluded that TBT may represent a substantial risk in some natural environments.
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Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Nesci, Salvatore
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Ventrella, Vittoria
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Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Ventrella, Vittoria
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Trombetti, Fabiana
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Pirini, Maurizio
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Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Pirini, Maurizio
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Borgatti, Anna Rosa
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Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Borgatti, Anna Rosa
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Pagliarani, Alessandra
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Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
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Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Nesci, Salvatore
;
Ventrella, Vittoria
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机构:
Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Ventrella, Vittoria
;
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Trombetti, Fabiana
;
Pirini, Maurizio
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h-index: 0
机构:
Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Pirini, Maurizio
;
Borgatti, Anna Rosa
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h-index: 0
机构:
Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy
Borgatti, Anna Rosa
;
Pagliarani, Alessandra
论文数: 0引用数: 0
h-index: 0
机构:
Univ Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, ItalyUniv Bologna, Dept Biochem G Moruzzi, Vet Biochem Sect, Via Tolara di Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy