Clonal spread and patient risk factors for acquisition of extensively drug-resistant Acinetobacter baumannii in a neonatal intensive care unit in Italy

被引：48

作者：

Zarrilli, R. ^{[1
,2
]}

Di Popolo, A. ^{[1
]}

Bagattini, M. ^{[1
]}

Giannouli, M. ^{[1
]}

Martino, D. ^{[1
]}

Barchitta, M. ^{[3
]}

Quattrocchi, A. ^{[3
]}

Iula, V. D. ^{[4
]}

de Luca, C. ^{[4
]}

Scarcella, A. ^{[5
]}

Triassi, M. ^{[1
]}

Agodi, A. ^{[3
]}

机构：

[1] Univ Naples Federico II, Dept Prevent Med Sci, Hyg Sect, I-80131 Naples, Italy

[2] CEINGE Adv Biotechnol, Naples, Italy

[3] Univ Catania, Dept GF Ingrassia, Catania, Italy

[4] Univ Naples Federico II, Dept Mol & Cellular Biol & Pathol L Califano, I-80131 Naples, Italy

[5] Univ Naples Federico II, Dept Paediat, I-80131 Naples, Italy

来源：

JOURNAL OF HOSPITAL INFECTION | 2012年 / 82卷 / 04期

关键词：

Acinetobacter baumannii; Extensively drug-resistant; Neonatal intensive care unit; Cross-transmission; Genotyping; Infection control; Risk factor analysis; MOLECULAR EPIDEMIOLOGY; PSEUDOMONAS-AERUGINOSA; INFECTION; OUTBREAK; COLONIZATION; EMERGENCE; SHOWS; ICU;

D O I：

10.1016/j.jhin.2012.08.018

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Aim: To report an outbreak of extensively drug-resistant (XDR) Acinetobacter baumannii in the neonatal intensive care unit (NICU) of an Italian university hospital. Patient risk profiles for acquisition of A. baumannii and measures used to control the outbreak are described. Methods: Antibiotic susceptibility of strains was evaluated by microdilution. Genotyping was performed by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing. Carbapenemase genes were analysed by polymerase chain reaction and DNA sequencing. A case-control study was designed to identify risk factors for acquisition of A. baumannii. Findings: A. baumannii was isolated from 22 neonates, six of whom were infected. One major PFGE type was identified, assigned to sequence type (ST) 2, corresponding to International Clone II; this was indistinguishable from isolates from the adult ICU in the same hospital. A. baumannii isolates were resistant to aminoglycosides, quinolones and classes of beta-lactam antibiotics, but were susceptible to tigecycline and colistin. Carbapenem resistance was associated with the presence of transposon Tn2006 carrying the bla(OxA)-23 gene. Length of NICU stay, length of exposure to A. baumannii, gestational age, use of invasive devices and length of exposure to invasive devices were significantly associated with acquisition of A. baumannii on univariate analysis, while length of exposure to central venous catheters and assisted ventilation were the only independent risk factors after multi-variate analysis. Conclusions: This XDR A. baumannii outbreak in an NICU was probably caused by intra-hospital transfer of bacteria via a colonized neonate whose mother was admitted to the adult ICU. Strengthened infection control measures were necessary to control the outbreak. (C) 2012 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.

引用

页码：260 / 265

页数：6