MicroRNA-16 inhibits migration and invasion via regulation of the Wnt/β-catenin signaling pathway in ovarian cancer

被引:26
作者
Li, Nan [1 ]
Yang, Liang [2 ]
Sun, Yanan [3 ]
Wu, Xiaohua [4 ]
机构
[1] Hebei Med Univ, Hosp 2, Dept Gynecol, Shijiazhuang 050017, Hebei, Peoples R China
[2] Hebei Med Univ, Hosp 2, Dept Neurosurg, Shijiazhuang 050017, Hebei, Peoples R China
[3] Bethune Int Peace Hosp Peoples Liberat Army, Dept Obstet & Gynecol, Shijiazhuang 050082, Hebei, Peoples R China
[4] Hebei Med Univ, Dept Obstet & Gynecol, 361 Zhongshan East Rd, Shijiazhuang 050017, Hebei, Peoples R China
关键词
microRNA-16; ovarian cancer; Wnt family member 3A; migration; invasion; EPITHELIAL-MESENCHYMAL TRANSITION; GENE-EXPRESSION; BIOMARKERS; PROGNOSIS;
D O I
10.3892/ol.2019.9923
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
As small non-coding RNA molecules, microRNAs (miRs) function in the regulation of tumorigenesis. Proliferation in ovarian cancer is considered to be associated with miR-16; however, the role of miR-16 in the migration and invasion of ovarian cancer cells remains unclear. The results of the present study demonstrated that miR-16 expression is downregulated in the ovarian cancer SKOV3 and OVCAR3 cell lines compared with that in normal ovarian epithelial cells (OECs). miR-16 overexpression inhibited the proliferation, migration and invasion of SKOV3 and OVCAR3 cells, and decreased the expression of matrix metallopeptidase (MMP)2 and MMP9. Additionally, miR-16 upregulated the expression of cadherin 1, an intercellular adhesion molecule, and downregulated the expression of some mesenchymal markers, including snail family transcriptional repressor 2, snail family transcriptional repressor 1, Vimentin, twist family BHLH transcription factor 1 and cadherin 2 in SKOV3 and OVCAR3 cells. Furthermore, it was indicated that miR-16 overexpression in SKOV3 and OVCAR3 cells resulted in a significant increase in anti-glycogen synthase kinase 3 beta expression and a decrease in the expression of Wnt family member 3A, beta-catenin, MYC proto-oncogene, BHLH transcription factor and cyclin D1 compared with the NC group. The results of the present study indicated that miR-16 exerts a suppressive effect on cell migration and invasion in ovarian cancer in vitro, through inactivation of the Wnt/beta-catenin signaling pathway. The data suggest that miR-16 may be a potential therapeutic agent for the treatment and prevention of ovarian cancer.
引用
收藏
页码:2631 / 2638
页数:8
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