Association analysis of IL10,TNF-α, and IL23R-IL12RB2 SNPs with Behcet's disease risk in estern Algeria

Objective: We have conducted the first study of the association of interleukin (IL)-10, tumor necrosis factor alpha (TNF-alpha), and IL23R-IL 12RB2 region single nucleotide polymorphisms (SNPs) with Behcet's disease (BD) in Western Algeria. Methods: A total of 51 BD patients and 96 unrelated controls from West region of Algeria were genotyped by direct sequencing for 11 SNPs including 2 SNPs from the IL10 promoter [c.-819T > C (rs1800871), c.-592A > C (rs1800872)1, 6 SNPs from the TNF-alpha promoter [c.1211T > C (rs1799964), c.-1043C > A (rs1800630), c.-1037C >T (rs1799724), c.-556G >A (rs1800750), c.-488G >A (rs1800629), and c.-418G >A (rs361525)1, and 3 SNPs from the IL23R-IL 12RB2 region [g.67747415A > C (rs12119179), g.67740092G >A (rs11209032), and g.67760140T > C (rs924080)]. Results: The minor alleles c.-819T and c.-592A were significantly associated with BD [odds ratio (OR) = 2.18; 95% confidence interval (Cl) 1.28-3.73, p= 0.0031; whereas, there was weaker association between TNF-alpha promoter SNPs or IL23R-IL 12RB2 region and disease risk. Conclusion: Unlike the TNF-alpha and the IL23R-IL12RB2 region SNPs, the two IL10 SNPs were strongly associated with BD. The -8191 and -592A alleles and the -819-R -819C1 and -592AA and -592CA genotypes seem to be highly involved in the risk of developing of BD in the population of Western Algeria.