Light regulates attachment, exopolysaccharide production, and nodulation in Rhizobium leguminosarum through a LOV-histidine kinase photoreceptor

被引:55
作者
Bonomi, Hernan R. [1 ]
Posadas, Diana M. [1 ]
Paris, Gaston [1 ]
del Carmen Carrica, Mariela [1 ]
Frederickson, Marcus [2 ]
Isabel Pietrasanta, Lia [3 ,4 ]
Bogomolni, Roberto A. [2 ]
Zorreguieta, Angeles [1 ,5 ]
Goldbaum, Fernando A. [1 ]
机构
[1] IIBBA Consejo Nacl Invest Cient & Tecn Argentina, Fdn Inst Leloir, Buenos Aires, DF, Argentina
[2] Univ Calif Santa Cruz, Dept Chem, Santa Cruz, CA 95064 USA
[3] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Ctr Microscopias Avanzadas, Buenos Aires, DF, Argentina
[4] Consejo Nacl Invest Cient & Tecn Argentina, Buenos Aires, DF, Argentina
[5] Univ Buenos Aires, FCEyN, Dept Quim Biol, Buenos Aires, DF, Argentina
基金
美国国家科学基金会;
关键词
BIOFILM FORMATION; GENES; PROTEINS; MOTILITY; FLAGELLA; SIGNALS; IDENTIFICATION; TRANSCRIPTION; CHEMOTAXIS; PHOTOCYCLE;
D O I
10.1073/pnas.1121292109
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Rhizobium leguminosarum is a soil bacterium that infects root hairs and induces the formation of nitrogen-fixing nodules on leguminous plants. Light, oxygen, and voltage (LOV)-domain proteins are blue-light receptors found in higher plants and many algae, fungi, and bacteria. The genome of R. leguminosarum bv. viciae 3841, a peanodulating endosymbiont, encodes a sensor histidine kinase containing a LOV domain at the N-terminal end (R-LOV-HK). R-LOV-HK has a typical LOV domain absorption spectrum with broad bands in the blue and UV-A regions and shows a truncated photocycle. Here we show that the R-LOV-HK protein regulates attachment to an abiotic surface and production of flagellar proteins and exopolysaccharide in response to light. Also, illumination of bacterial cultures before inoculation of pea roots increases the number of nodules per plant and the number of intranodular bacteroids. The effects of light on nodulation are dependent on a functional lov gene. The results presented in this work suggest that light, sensed by R-LOV-HK, is an important environmental factor that controls adaptive responses and the symbiotic efficiency of R. leguminosarum.
引用
收藏
页码:12135 / 12140
页数:6
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