Dengue virus strain DEN2 16681 utilizes a specific glycochain of syndecan-2 proteoglycan as a receptor

被引：40

作者：

Okamoto, Kenta ^{[1
,2
]}

Kinoshita, Hitomi ^{[1
,2
]}

Parquet, Maria del Carmen ^{[1
,2
]}

Raekiansyah, Muhareva ^{[1
,2
]}

Kimura, Daisuke ^{[2
,3
]}

Yui, Katsuyuki ^{[2
,3
]}

Islam, Mohammed Alimul ^{[4
]}

Hasebe, Futoshi ^{[1
,2
]}

Morita, Kouichi ^{[1
,2
]}

机构：

[1] Nagasaki Univ, Dept Virol, Inst Trop Med, Nagasaki 852, Japan

[2] Global COE Program, Nagasaki, Japan

[3] Nagasaki Univ, Div Immunol, Dept Mol Microbiol & Immunol, Grad Sch Biomed Sci, Nagasaki 852, Japan

[4] Bangladesh Agr Univ, Dept Microbiol & Hyg, Fac Vet Sci, Mymensingh 2202, Bangladesh

来源：

JOURNAL OF GENERAL VIROLOGY | 2012年 / 93卷

基金：

日本学术振兴会;

关键词：

HEPARAN-SULFATE PROTEOGLYCANS; JAPANESE ENCEPHALITIS-VIRUS; HUMAN DENDRITIC CELLS; HUMAN-BONE-MARROW; ENVELOPE PROTEIN; MONONUCLEAR PHAGOCYTES; INDUCED HEMORRHAGE; MOUSE MODEL; INFECTION; BINDING;

D O I：

10.1099/vir.0.037853-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Dengue virus (DENV) causes fever and severe haemorrhagic symptoms in humans. The DEN2 16681 strain, derived from a dengue haemorrhagic fever patient, has been widely used in studies related to DENV pathogenesis, such as mouse and non-human primate haemorrhagic models and human vascular endothelial-cell permeability. To clarify the entry mechanism of the 1 6681 strain, we characterized a novel cell receptor for this strain. Our two major findings were as follows: firstly, the SDC2 membrane protein was an effective DEN2 16681 receptor in a cloned K562 cell line. Secondly, a heparan sulfate (HS) glycochain (of four glycochains in SDC2) is the specific binding site of DENV and seems to be involved in tissue-culture adaptation. Our findings present an entry mechanism that could be implicated for DENV adaptation and HS-mediated DENV infection.

引用

页码：761 / 770

页数：10

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