Formation of a chiral acetoinic compound from diacetyl by Escherichia coli expressing meso-2,3-butanediol dehydrogenase

被引：15

作者：

Ui, S ^{[1
]}

Mimura, A

Ohkuma, M

Kudo, T

机构：

[1] Yamanashi Univ, Fac Engn, Dept Appl Chem & Biotechnol, Yamanashi 4008511, Japan

[2] Inst Phys & Chem Res, Microbiol Lab, Wako, Saitama, Japan

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1999年 / 28卷 / 06期

关键词：

D O I：

10.1046/j.1365-2672.1999.00560.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

L-Acetoin (L-AC) was produced from diacetyl (DA) by Escherichia coli JM109/pBUD119 containing the meso-2,3-butanediol dehydrogenase (D-AC forming) gene. However, when the strain was cultured in the presence of isopropyl-beta-D-thiogalacto-pyranoside, the enzyme formed catalysed not only D-AC but also L-AC. L-AC was further reduced to L-2,3-butanediol (BD). The yield of L-AC or L-BD from DA (3 g l(-1)) was about 70% (w/w).

引用

页码：457 / 460

页数：4

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