The determination of in vivo human tissue optical properties and absolute chromophore concentrations using spatially resolved steady-state diffuse reflectance spectroscopy

被引:339
作者
Doornbos, RMP
Lang, R
Aalders, MC
Cross, FW
Sterenborg, HJCM
机构
[1] Univ Amsterdam, Acad Med Ctr, Ctr Laser, NL-1105 AZ Amsterdam, Netherlands
[2] Royal London Hosp, Dept Vasc Surg, London E1 1BB, England
[3] Univ Rotterdam Hosp, Dr Daniel Den Hoed Canc Ctr, Dept Radiotherapy, NL-3008 AE Rotterdam, Netherlands
关键词
D O I
10.1088/0031-9155/44/4/012
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
A method is described for measuring optical properties and deriving chromophore concentrations from diffuse reflection measurements at the surface of a turbid medium. The method uses a diffusion approximation model for the diffuse reflectance, in combination with models for the absorption and scattering coefficients. An optical fibre-based set-up, capable of measuring nine spectra from 400 to 1050 nm simultaneously, is used to test the method experimentally. Results of the analyses of phantom and in vivo measurements are presented. These demonstrate that in the wavelength range from 600 to 900 nm, tissue scattering can be described as a simple power dependence of the wavelength and that the tissue absorption can be accurately described by the addition of water, oxy- and deoxyhaemoglobin absorption.
引用
收藏
页码:967 / 981
页数:15
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