Cytokines in osteoblast-conditioned medium promote the migration of breast cancer cells

被引：14

作者：

Chen, Xiaojia ^{[1
,2
]}

Lu, Jia ^{[1
,2
]}

Ji, Yuhua ^{[1
,2
]}

Hong, An ^{[1
,2
]}

Xie, Qiuling ^{[1
,2
]}

机构：

[1] Guangdong Prov Key Lab Bioengn Med, Guangzhou 510632, Guangdong, Peoples R China

[2] China Natl Engn Res Ctr Genet Med, Guangzhou 510632, Guangdong, Peoples R China

来源：

TUMOR BIOLOGY | 2014年 / 35卷 / 01期

关键词：

Cytokines; Osteoblast-conditioned medium (OCM); Breast cancer; Bone microenvironment; NF-KAPPA-B; HUMAN-MELANOMA CELLS; INTERLEUKIN-8; EXPRESSION; UP-REGULATION; BONE; GROWTH; MOTILITY; TUMORIGENICITY; METASTASIS; SUPPRESSES;

D O I：

10.1007/s13277-013-1109-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Bone is one of the most common metastatic sites for breast cancer. In this study, we observed a promoting effect of osteoblast-conditioned medium (OCM) on the migration of MCF-7, a noninvasive cell line of breast cancer cells. Cytokine antibody array was used to compare the cytokines of OCM with the conditioned medium of non-differentiated osteoblast cells, which consequently revealed factors related to migration, such as IL8, IL6, CSF2 (G-CSF), CSF3 (GM-CSF), and TNFRSF11B (osteoprotegerin). The expression of genes related to migration was also estimated with a PCR array, which showed that 9 genes were upregulated and 26 genes downregulated. Moreover, activated p38, ERK, and AKT pathways were found in the OCM treatment group. This finding indicated the migration ability of breast cancer cells, which move toward the bone depending on the presence of specific cytokines in its surrounding microenvironment.

引用

页码：791 / 798

页数：8

共 47 条

[11] Granulocyte Macrophage Colony-Stimulating Factor Inhibits Breast Cancer Growth and Metastasis by Invoking an Anti-Angiogenic Program in Tumor-Educated Macrophages [J].

Eubank, Tim D. ;

Roberts, Ryan D. ;

Khan, Mahmood ;

Curry, Jennifer M. ;

Nuovo, Gerard J. ;

Kuppusamyl, Periannan ;

Marsh, Clay B. .

CANCER RESEARCH, 2009, 69 (05) :2133-2140

[12] Osteoblast-derived TGF-β1 stimulates IL-8 release through AP-1 and NF-κB in human cancer cells [J].

Fong, Yi-Chin ;

Maa, Ming-Chei ;

Tsai, Fuu-Jen ;

Chen, Wen-Chi ;

Lin, Jaung-Geng ;

Jeng, Long-Bin ;

Yang, Rong-Sen ;

Fu, Wen-Mei ;

Tang, Chih-Hsin .

JOURNAL OF BONE AND MINERAL RESEARCH, 2008, 23 (06) :961-970

[13] The hallmarks of cancer [J].

Hanahan, D ;

Weinberg, RA .

CELL, 2000, 100 (01) :57-70

[14]

Inoue K, 2000, CANCER RES, V60, P2290

[15] Insulin-like Growth Factor Binding Protein 7 Mediates Glioma Cell Growth and Migration [J].

Jiang, Wei ;

Xiang, Cunli ;

Cazacu, Simona ;

Brodie, Chaya ;

Mikkelsen, Tom .

NEOPLASIA, 2008, 10 (12) :1335-1342

[16] Mechanisms of osteolytic bone metastases in breast carcinoma [J].

Käkönen, SM ;

Mundy, GR .

CANCER, 2003, 97 (03) :834-839

[17]

KOHN EC, 1993, INT J CANCER, V53, P968

[18] Osteoblasts-derived BMP-2 enhances the motility of prostate cancer cells via activation of integrins [J].

Lai, Tzu-Hsiu ;

Fong, Yi-Chin ;

Fu, Wen-Mei ;

Yang, Rong-Sen ;

Tang, Chih-Hsin .

PROSTATE, 2008, 68 (12) :1341-1353

[19]

LEI X, 2004, CLIN CANCER RES, V10, P701

[20] Transient transfection factors for high-level recombinant protein production in suspension cultured mammalian cells [J].

Liu, Chaoting ;

Dalby, Brian ;

Chen, Weixing ;

Kilzer, Jennifer M. ;

Chiou, Henry C. .

MOLECULAR BIOTECHNOLOGY, 2008, 39 (02) :141-153

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