Cytokines in osteoblast-conditioned medium promote the migration of breast cancer cells

被引：14
作者：
Chen, Xiaojia [1 ,2 ]
Lu, Jia [1 ,2 ]
Ji, Yuhua [1 ,2 ]
Hong, An [1 ,2 ]
Xie, Qiuling [1 ,2 ]
机构：
[1] Guangdong Prov Key Lab Bioengn Med, Guangzhou 510632, Guangdong, Peoples R China
[2] China Natl Engn Res Ctr Genet Med, Guangzhou 510632, Guangdong, Peoples R China
来源：
TUMOR BIOLOGY | 2014年 / 35卷 / 01期
关键词：
Cytokines; Osteoblast-conditioned medium (OCM); Breast cancer; Bone microenvironment; NF-KAPPA-B; HUMAN-MELANOMA CELLS; INTERLEUKIN-8; EXPRESSION; UP-REGULATION; BONE; GROWTH; MOTILITY; TUMORIGENICITY; METASTASIS; SUPPRESSES;
D O I：
10.1007/s13277-013-1109-0
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Bone is one of the most common metastatic sites for breast cancer. In this study, we observed a promoting effect of osteoblast-conditioned medium (OCM) on the migration of MCF-7, a noninvasive cell line of breast cancer cells. Cytokine antibody array was used to compare the cytokines of OCM with the conditioned medium of non-differentiated osteoblast cells, which consequently revealed factors related to migration, such as IL8, IL6, CSF2 (G-CSF), CSF3 (GM-CSF), and TNFRSF11B (osteoprotegerin). The expression of genes related to migration was also estimated with a PCR array, which showed that 9 genes were upregulated and 26 genes downregulated. Moreover, activated p38, ERK, and AKT pathways were found in the OCM treatment group. This finding indicated the migration ability of breast cancer cells, which move toward the bone depending on the presence of specific cytokines in its surrounding microenvironment.
引用
收藏
页码：791 / 798
页数：8
相关论文
共 47 条
[1]   Insulin like growth factor binding protein-7 reduces growth of human breast cancer cells and xenografted tumors [J].
Amemiya, Y. ;
Yang, W. ;
Benatar, T. ;
Nofech-Mozes, S. ;
Yee, A. ;
Kahn, H. ;
Holloway, C. ;
Seth, Arun .
BREAST CANCER RESEARCH AND TREATMENT, 2011, 126 (02) :373-384
[2]   Interleukin-8 is a molecular determinant of androgen independence and progression in prostate cancer [J].
Araki, Shinako ;
Omori, Yohei ;
Lyn, Dominic ;
Singh, Rajendra K. ;
Meinbach, David M. ;
Sandman, Yekutiel ;
Lokeshwar, Vinata B. ;
Lokeshwar, Bal L. .
CANCER RESEARCH, 2007, 67 (14) :6854-6862
[3]   Increased serum interleukin-8 in patients with early and metastatic breast cancer correlates with early dissemination and survival [J].
Benoy, IH ;
Salgado, R ;
Van Dam, P ;
Geboers, K ;
Van Marck, E ;
Scharpé, S ;
Vermeulen, PB ;
Dirix, LY .
CLINICAL CANCER RESEARCH, 2004, 10 (21) :7157-7162
[4]   Osteoblast-conditioned medium promotes proliferation and sensitizes breast cancer cells to imatinib treatment [J].
Brama, Marina ;
Basciani, Sabrina ;
Cherubini, Sara ;
Mariani, Stefania ;
Migliaccio, Silvia ;
Arizzi, Mario ;
Rosano, Giuseppe ;
Spera, Giovanni ;
Gnessi, Lucio .
ENDOCRINE-RELATED CANCER, 2007, 14 (01) :61-72
[5]   The role of interleukin-8 and its receptors in gliomagenesis and tumoral angiogenesis [J].
Brat, DJ ;
Bellail, AC ;
Van Meir, EG .
NEURO-ONCOLOGY, 2005, 7 (02) :122-133
[6]  
BRONNER F, 2009, BONE CANC, V5, P141
[7]   DEXAMETHASONE REGULATES IL-1-BETA AND TNF-ALPHA-INDUCED INTERLEUKIN-8 PRODUCTION IN HUMAN BONE-MARROW STROMAL AND OSTEOBLAST-LIKE CELLS [J].
CHAUDHARY, LR ;
AVIOLI, LV .
CALCIFIED TISSUE INTERNATIONAL, 1994, 55 (01) :16-20
[8]   KiSS1 Suppresses TNFα-Induced Breast Cancer Cell Invasion Via an Inhibition of RhoA-Mediated NF-κB Activation [J].
Cho, Sung-Gook ;
Li, Dali ;
Stafford, Lewis J. ;
Luo, Jian ;
Rodriguez-Villanueva, Melissa ;
Wang, Ying ;
Liu, Mingyao .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2009, 107 (06) :1139-1149
[9]   KAI1/CD82 is a novel target of estrogen receptor-mediated gene repression and downregulated in primary human breast cancer [J].
Christgen, Matthias ;
Bruchhardt, Henriette ;
Ballmaier, Matthias ;
Krech, Till ;
Laenger, Florian ;
Kreipe, Hans ;
Lehmann, Ulrich .
INTERNATIONAL JOURNAL OF CANCER, 2008, 123 (10) :2239-2246
[10]   The potential role of neutrophils in promoting the metastatic phenotype of tumors releasing interleukin-8 [J].
De Larco, JE ;
Wuertz, BRK ;
Furcht, LT .
CLINICAL CANCER RESEARCH, 2004, 10 (15) :4895-4900
12345