First Molecular Characterization of Cryptosporidium spp. Infecting Buffalo Calves in Brazil

被引：12

作者：

Aquino, Monally C. C. ^{[1
]}

Widmer, Giovanni ^{[2
]}

Zucatto, Anaiza S. ^{[1
]}

Viol, Milena A. ^{[1
]}

Inacio, Sandra V. ^{[1
]}

Nakamura, Alex A. ^{[1
]}

Coelho, Willian M. D. ^{[3
]}

Perri, Silvia H. V. ^{[1
]}

Meireles, Marcelo V. ^{[1
]}

Bresciani, Katia D. S. ^{[1
]}

机构：

[1] Univ Estadual Paulista, UNESP, Fac Med Vet Aracatuba, Sao Paulo, Brazil

[2] Tufts Univ, Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA USA

[3] Univ Estadual Paulista, UNESP, Fac Ciencias Agr & Vet, Sao Paulo, Brazil

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 2015年 / 62卷 / 05期

基金：

巴西圣保罗研究基金会;

关键词：

Cryptosporidiosis; Cryptosporidium ryanae; genotype; nested-PCR; water buffaloes; SP APICOMPLEXA CRYPTOSPORIDIIDAE; BUBALUS-BUBALIS; WATER-BUFFALO; ZOONOTIC TRANSMISSION; SEQUENCE ALIGNMENT; DAIRY CALVES; RISK-FACTORS; BOS-TAURUS; CATTLE; GIARDIA;

D O I：

10.1111/jeu.12223

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae, which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes.

引用

页码：657 / 661

页数：5

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