Positive diversity-functioning relationships in model communities of methanotrophic bacteria

被引:27
作者
Schnyder, Elvira [1 ]
Bodelier, Paul L. E. [2 ]
Hartmann, Martin [3 ]
Henneberger, Ruth [4 ,6 ]
Niklaus, Pascal A. [1 ,5 ]
机构
[1] Univ Zurich, Dept Evolutionary Biol & Environm Studies, Winterthurerstr 190, CH-8057 Zurich, Switzerland
[2] Netherlands Inst Ecol NIOO KNAW, Dept Microbial Ecol, Droevendaalsesteeg 10, NL-6708 PB Wageningen, Netherlands
[3] Swiss Fed Res Inst WSL, Forest Soils & Biogeochem, Zurcherstr 111, CH-8903 Birmensdorf, Switzerland
[4] Swiss Fed Inst Technol, Inst Biogeochem & Pollutant Dynam, Univ Str 16, CH-8092 Zurich, Switzerland
[5] Univ Zurich, Zurich Res Prior Program Global Change & Biodiver, Winterthurerstr 190, CH-8057 Zurich, Switzerland
[6] Swiss Fed Inst Technol, Inst Mol Hlth Sci, Ramistr 101, CH-8092 Zurich, Switzerland
基金
瑞士国家科学基金会;
关键词
biodiversity; ecosystem function; mechanisms; methane oxidation; methanotrophic bacteria; niche complementarity; overyielding; soil ecology; ECOSYSTEM FUNCTION RELATIONSHIP; METHANE-OXIDIZING BACTERIA; COMPLETE GENOME SEQUENCE; ARCTIC WETLAND SOIL; MICROBIAL DIVERSITY; SP NOV; SPECIES-DIVERSITY; BIODIVERSITY EXPERIMENTS; AEROBIC METHANOTROPHS; GRASSLAND ECOSYSTEMS;
D O I
10.1002/ecy.2138
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Biodiversity enhances ecosystem functions such as biomass production and nutrient cycling. Although the majority of the terrestrial biodiversity is hidden in soils, very little is known about the importance of the diversity of microbial communities for soil functioning. Here, we tested effects of biodiversity on the functioning of methanotrophs, a specialized group of soil bacteria that plays a key role in mediating greenhouse gas emissions from soils. Using pure strains of methanotrophic bacteria, we assembled artificial communities of different diversity levels, with which we inoculated sterile soil microcosms. To assess the functioning of these communities, we measured methane oxidation by gas chromatography throughout the experiment and determined changes in community composition and community size at several time points by quantitative PCR and sequencing. We demonstrate that microbial diversity had a positive overyielding effect on methane oxidation, in particular at the beginning of the experiment. This higher assimilation of CH4 at high diversity translated into increased growth and significantly larger communities towards the end of the study. The overyielding of mixtures with respect to CH4 consumption and community size were positively correlated. The temporal CH4 consumption profiles of strain monocultures differed, raising the possibility that temporal complementarity of component strains drove the observed community-level strain richness effects; however, the community niche metric we derived from the temporal activity profiles did not explain the observed strain richness effect. The strain richness effect also was unrelated to both the phylogenetic and functional trait diversity of mixed communities. Overall, our results suggest that positive biodiversity-ecosystem-function relationships show similar patterns across different scales and may be widespread in nature. Additionally, biodiversity is probably also important in natural methanotrophic communities for the ecosystem function methane oxidation. Therefore, maintaining soil conditions that support a high diversity of methanotrophs may help to reduce the emission of the greenhouse gas methane.
引用
收藏
页码:714 / 723
页数:10
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