Competitive Binding of Abasic Site-Binding Ligands and Masking Ligands to DNA Duplexes for the Analysis of Single-Base Mutation

被引：9

作者：

Sato, Yusuke ^{[1
]}

Kageyama, Tomoe ^{[1
]}

Nishizawa, Seiichi ^{[1
]}

Teramae, Norio ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Sci, Dept Chem, Aoba Ku, Sendai, Miyagi 9808578, Japan

来源：

ANALYTICAL SCIENCES | 2013年 / 29卷 / 01期

关键词：

OPPOSITE AP SITES; SELECTIVE BINDING; NUCLEOTIDE POLYMORPHISMS; FLUORESCENT LIGANDS; CYTOSINE OPPOSITE; ADENINE OPPOSITE; RECOGNITION; ALLOXAZINE; AFFINITY; TARGETS;

D O I：

10.2116/analsci.29.15

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe a competitive binding assay for the analysis of single-base mutation by using abasic site (AP site)-specific binding fluorescent ligands and masking ligands bound to the AP site in DNA duplexes. 2-Amino-5,6,7-trimethy1-1,8-naphthyridine (ATMND) can strongly bind to not only cytosine (C), but also thymine (T) opposite an AP site in the DNA duplexes (K-11/10(7) M-1: C, 15; T, 7.0). By contrast, in the presence of lumichrome (Lch) as a masking ligand, ATMND shows a clear binding selectivity for C over T (K-11/10(7) M-1: C, 7.0; T, 0.57), which is ascribed to the effective masking effect of Lch to the ATMND-T interaction in the AP site-containing DNA duplexes. It was shown that the competitive binding of ATMND and Lch to the AP site allowed the highly selective detection of C-related single-base mutation in DNAs amplified by polymerase chain reaction.

引用

页码：15 / 19

页数：5

共 35 条

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