Voluntary wheel running and testosterone replacement increases heart angiogenesis through miR-132 in castrated diabetic rats

被引：6
作者：
Chodari, L. [1 ,2 ]
Dariushnejad, H. [3 ]
Ghorbanzadeh, V [4 ]
机构：
[1] Urmia Univ Med Sci, Dept Med Physiol, Orumiyeh, Iran
[2] Urmia Univ Med Sci, Neurophysiol Res Ctr, Orumiyeh, Iran
[3] Lorestan Univ Med Sci, Fac Med, Dept Med Biotechnol, Khorramabad, Iran
[4] Lorestan Univ Med Sci, Razi Herbal Med Res Ctr, End Razi Ave, Khorramabad 6813833946, Iran
来源：
PHYSIOLOGY INTERNATIONAL | 2019年 / 106卷 / 01期
关键词：
testosterone; voluntary exercise; miR-132; angiogenesis; diabetes; ENHANCING EXPRESSION; OXIDATIVE STRESS; EXERCISE; SEX; MEN; MORTALITY; IMPROVES; VEGF;
D O I：
10.1556/2060.106.2019.06
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Objective: Low levels of testosterone in men with diabetes are associated with cardiovascular complications. We investigated the effect of testosterone and voluntary exercise on heart angiogenesis in castrated diabetic rats. Methods: Sixty-three diabetic rats were treated with testosterone 2 mg/kg/day or voluntary exercise alone or combination of these two for 6 weeks. At the end of the study, heart tissue samples were collected and used for CD31 detection by immunohistochemical method and determination of miR-132 levels. Results: miR-132 levels and CD31 of heart tissue were higher after testosterone administration and in the voluntary exercise group in diabetic rats after 6 weeks. Combination of testosterone and voluntary exercise had synergistic effect on angiogenesis and miR-132 level. In castrated diabetic rats, there were significantly lower levels of miR-132 and CD31 in heart tissue compared to the diabetic group, whereas testosterone and exercise reversed these effects. In addition, testosterone supplementation plus exercise had an additive effect on miR-132 levels and CD31 in castrated diabetic rats. Conclusions: It was concluded that castration in rats leads to reduced miR-132 levels and subsequently decreased angiogenesis in diabetes. Testosterone plus voluntary exercise improved angiogenesis possibly through enhancement of miR-132 levels in heart of castrated diabetic rats.
引用
收藏
页码：48 / 58
页数：11
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