Evaluation of microRNA Gene Polymorphisms in Liver Transplant Patients with Hepatocellular Carcinoma

被引：1

作者：

Dehbidi, Sahar ^{[1
]}

Farokhizadeh, Zhaleh ^{[1
]}

Karimi, Mohammad Hossein ^{[2
]}

Afshari, Afsoon ^{[2
]}

Behmanesh, Mehrdad ^{[3
]}

Sanati, Mohammad Hossein ^{[4
]}

Geramizadeh, Bita ^{[2
]}

Yaghobi, Ramin ^{[2
]}

机构：

[1] Nour Danesh Inst Higher Educ, Fac Biol Sci, Dept Biol, Esfahan, Iran

[2] Shiraz Univ Med Sci, Shiraz Transplant Res Ctr, Shiraz, Iran

[3] Tarbiat Modares Univ, Fac Biol Sci, Dept Genet, Tehran, Iran

[4] Natl Inst Genet Engn & Biotechnol, Dept Med Genet, Tehran, Iran

来源：

HEPATITIS MONTHLY | 2020年 / 20卷 / 07期

关键词：

Hepatocellular Carcinoma; MicroRNA; Liver Transplantation; Rejection; Polymorphism; TURKISH POPULATION; FUNCTIONAL POLYMORPHISM; EXPRESSION PATTERNS; HUMAN CANCER; ASSOCIATION; RISK; INFECTION; SUSCEPTIBILITY; MIR-499A-GREATER-THAN-G; CONTRIBUTES;

D O I：

10.5812/hepatmon.102690

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background: Genetic polymorphism in the miRNA sequence might alter miRNA expression and/or maturation, which is associated with the development and progression of hepatocellular carcinoma (HCC) in liver transplant patients. Objectives: Therefore, the prevalence of miRNA-146a G > C (rs2910164), miRNA-499A > G (rs3746444), miRNA-149C > T (rs2292832), and miRNA-196a-2 C > T (rs11614913) gene polymorphisms was evaluated in liver recipients with HCC with or without experiencing graft rejection. Methods: In a cross-sectional study, tissue samples were collected from 60 HCC patients who underwent liver transplant surgery at Namazi Hospital, Shiraz, Iran, in 2013 - 2015. A control group consisting of 120 individuals was randomly selected, as well. The genomic DNA was extracted from collected tissues and blood samples. The miRNA-146a (rs2910164), miRNA-499 (rs3746444), miRNA-149 (rs2292832), and miRNA-196a-2 (rs11614913) gene polymorphisms were evaluated in patients with HCC using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Results: The CC genotype and C allele of the miRNA-146a (rs2910164) polymorphism were significantly associated with the increased risk of transplant rejection in patients with HCC (P = 0.05 and P = 0.05, respectively). The CC genotype and C allele of the miRNA-146a (rs2910164) were also significantly more frequent in male liver transplant patients who experienced acute rejection than in non-rejected ones (P = 0.05 and P = 0.03, respectively). However, no significant association was found between the genotypes and alleles of miRNA-499 (rs3746444), miRNA-149 (rs2292832), and miRNA-196a-2 (rs11614913) polymorphisms and HCC outcomes in liver transplant recipients. Conclusions: The importance of the CC genotype and C allele of the miRNA-146a (rs2910164) polymorphism in increasing the risk of transplant rejection was confirmed, but it needs further studies in larger populations.

引用

页码：1 / 9

页数：9

共 50 条

[21] Low Frequency of Cancer-Predisposition Gene Mutations in Liver Transplant Candidates with Hepatocellular Carcinoma [J].

Horackova, Klara ;

Frankova, Sona ;

Zemankova, Petra ;

Nehasil, Petr ;

Cerna, Marta ;

Neroldova, Magdalena ;

Otahalova, Barbora ;

Kral, Jan ;

Hovhannisyan, Milena ;

Stranecky, Viktor ;

Zima, Tomas ;

Safarikova, Marketa ;

Kalousova, Marta ;

CZECANCA Consortium, C. Z. E. C. A. N. C. A. ;

Novotny, Jan ;

Sperl, Jan ;

Borecka, Marianna ;

Jelinkova, Sandra ;

Vocka, Michal ;

Janatova, Marketa ;

Kleiblova, Petra ;

Kleibl, Zdenek ;

Jirsa, Milan ;

Soukupova, Jana .

CANCERS, 2023, 15 (01)

[22] Four microRNA gene polymorphisms are associated with Iraqi patients with colorectal cancer [J].

Jameel, Zahraa Isam .

EGYPTIAN JOURNAL OF MEDICAL HUMAN GENETICS, 2024, 25 (01)

[23] Prognostic Impact of Intraoperative Blood Loss in Liver Transplant Patients with Advanced Hepatocellular Carcinoma [J].

Kornberg, Arno ;

Witt, Ulrike ;

Kornberg, Jennifer ;

Ceyhan, Gueralp O. ;

Mueller, Katharina ;

Friess, Helmut ;

Thrum, Katharina .

ANTICANCER RESEARCH, 2016, 36 (10) :5355-5364

[24] Salvage Liver Transplant versus Primary Liver Transplant for Patients with Hepatocellular Carcinoma [J].

Yadav, Dipesh Kumar ;

Chen, Wei ;

Bai, Xueli ;

Singh, Alina ;

Li, Guogang ;

Ma, Tao ;

Yu, Xiazhen ;

Xiao, Zhi ;

Huang, Bingfeng ;

Liang, Tingbo .

ANNALS OF TRANSPLANTATION, 2018, 23 :524-545

[25] Promoter polymorphisms of pri-miR-34b/c are associated with hepatocellular carcinoma [J].

Son, Myung Su ;

Jang, Moon Ju ;

Jeon, Young Joo ;

Kim, Won Hee ;

Kwon, Chang-Il ;

Ko, Kwang Hyun ;

Park, Pil Won ;

Hong, Sung Pyo ;

Rim, Kyu Sung ;

Kwon, Sung Won ;

Hwang, Seong Gyu ;

Kim, Nam Keun .

GENE, 2013, 524 (02) :156-160

[26] MiRNA Polymorphisms and Hepatocellular Carcinoma Susceptibility: A Systematic Review and Network Meta-Analysis [J].

Zhang, Qimeng ;

Xu, Xueying ;

Wu, Mingcheng ;

Qin, Tiantian ;

Wu, Shaoning ;

Liu, Hongbo .

FRONTIERS IN ONCOLOGY, 2021, 10

[27] Risk factors for liver transplant waitlist dropout in patients with hepatocellular carcinoma [J].

Park, Sang-Jae ;

Freise, Chris E. ;

Hirose, Ryutaro ;

Kerlan, Robert K. ;

Yao, Francis Y. ;

Roberts, John P. ;

Vagefi, Parsia A. .

CLINICAL TRANSPLANTATION, 2012, 26 (04) :E359-E364

[28] Hepatocellular Carcinoma Patients Are Advantaged in the Current Liver Transplant Allocation System [J].

Washburn, K. ;

Edwards, E. ;

Harper, A. ;

Freeman, R. B. .

AMERICAN JOURNAL OF TRANSPLANTATION, 2010, 10 (07) :1643-1648

[29] MicroRNA Gene Polymorphisms and Environmental Factors Increase Patient Susceptibility to Hepatocellular Carcinoma [J].

Chu, Yin-Hung ;

Hsieh, Ming-Ju ;

Chiou, Hui-Ling ;

Liou, Yi-Sheng ;

Yang, Chen-Chieh ;

Yang, Shun-Fa ;

Kuo, Wu-Hsien .

PLOS ONE, 2014, 9 (02)

[30] Impacts of TCF7L2 gene polymorphisms on the susceptibility of hepatogenous diabetes and hepatocellular carcinoma in cirrhotic patients [J].

Ling, Qi ;

Dong, Fengqin ;

Geng, Lei ;

Liu, Zhikun ;

Xie, Haiyang ;

Xu, Xiao ;

Zheng, Shusen .

GENE, 2013, 522 (02) :214-218

← 1 2 3 4 5 →