DNA aptamer generation by ExSELEX using genetic alphabet expansion with a mini-hairpin DNA stabilization method

被引：35

作者：

Hirao, Ichiro ^{[1
]}

Kimoto, Michiko ^{[1
]}

Lee, Kyung Hyun ^{[1
]}

机构：

[1] Inst Bioengn & Nanotechnol, 31 Biopolis Way,Nanos 09-01, Singapore 138669, Singapore

来源：

BIOCHIMIE | 2018年 / 145卷

基金：

新加坡国家研究基金会;

关键词：

Aptamer; ExSELEX; Genetic alphabet expansion; Unnatural base pair; Mini-hairpin DNA; UNNATURAL BASE-PAIR; IN-VITRO SELECTION; SEMISYNTHETIC ORGANISM; PHARMACEUTICAL APPLICATIONS; EXPONENTIAL ENRICHMENT; LABORATORY EVOLUTION; SYSTEMATIC EVOLUTION; STABLE STRUCTURE; RNA; LIGANDS;

D O I：

10.1016/j.biochi.2017.09.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel aptamer generation method to greatly augment the affinity and stability of DNA aptamers was developed by genetic alphabet expansion combined with mini-hairpin DNA technology. The genetic alphabet expansion increases the physicochemical and structural diversities of DNA aptamers by introducing extra components, unnatural bases, as a fifth base, allowing for the enhancement of DNA aptamer affinities. Furthermore, the mini-hairpin DNA technology stabilizes DNA aptamers against nuclease digestion and thermal denaturation, by introducing an extraordinarily stable mini-hairpin DNA containing a GCGAAGC sequence. This novel method provides stabilized high-affinity DNA aptamers for diagnostic and therapeutic applications. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved.

引用

页码：15 / 21

页数：7