βig-h3 Interacts with α3β1 Integrin to Promote Adhesion and Migration of Human Hepatoma Cells

被引：27
作者：
Tang, Juan
Wu, Ya-Mei
Zhao, Pu
Jiang, Jian-Li [1 ]
Chen, Zhi-Nan
机构：
[1] Fourth Mil Med Univ, Cell Engn Res Ctr, Xian 710032, Peoples R China
来源：
EXPERIMENTAL BIOLOGY AND MEDICINE | 2009年 / 234卷 / 01期
基金：
中国国家自然科学基金;
关键词：
beta ig-h3; hepatoma cells; metastasis; signal pathway; alpha; 3; beta; 1; integrin; HEPATOCELLULAR-CARCINOMA; PROTEIN; KINASE; GROWTH; IDENTIFICATION; PROLIFERATION; METASTASIS; DOMAINS; MOTIFS; GENE;
D O I：
10.3181/0806-RM-187
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
beta ig-h3 is a TGF-induced extracellular matrix (ECM) protein. Our previous evidence suggests that beta ig-h3 may promote adhesion and invasion potential of human hepatoma cells, but the mechanism underlying this process is still unknown. The present study identifies a pivotal role for molecules of the beta ig-h3 signal transduction pathway. We demonstrated that beta ig-h3 co-immunoprecipitated with alpha 3 beta 1 integrin in human 7721 hepatoma cells. The addition of alpha 3 beta 1 integrin antibodies inhibited the elevated adhesion and migration in beta ig-h3-overexpressing 7721 cells, but not in beta ig-h3 siRNA transfected 7721 cells. The expression and activity of integrin downstream molecules FAK and paxillin show a positive correlation with beta ig-h3 expression in different human hepatoma cells. Levels of focal adhesions and stress fibers were decreased in beta ig-h3 siRNA transfected 7721 cells. We suggest that by interaction with alpha 3 beta 1 integrin, beta ig-h3 activates FAK-paxillin signaling linkage, leads to cytoskeleton reorganization, and thus enhances the metastatic potentials of human hepatoma cells. Exp Biol Med 234:35-39, 2009
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页码：35 / 39
页数：5
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