Helicobacter pylori damages human gallbladder epithelial cells in vitro

AIM: To study the mechanism by which Helicobacter pylori (H pylori) damages human gallbladder epithelial cells (HGBEC). METHODS: H pylori isolated from gallbladder were cultured in a liquid medium, Different concentration supernatants and sonicated extracts of H pylori cells were then added to HGBEC in a primary culture. The morphological changes in HGBEC as well as changes in the levels of alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and glutamyltransferase (GGT) were measured. RESULTS: According to the culture curve of HGBEC, it was convenient to study the changes in HGBEC by adding H pylori sonicated extracts and H pylori culture supernatants. Both H pylori sonicated extracts and H pylori culture supernatants had a significant influence on HGBEC morphology, i.e. HGBEC grew more slowly, their viability decreased and their detachment increased. Furthermore, HGBEC ruptured and died. The levels of ALP (33.84 +/- 6.00 vs 27.01 +/- 4.67, P < 0.05), LDH (168.37 +/- 20.84 vs 55.51 +/- 17.17, P < 0.01) and GGT (42.01 +/- 6.18 vs 25.34 +/- 4.33, P < 0.01) significantly increased in the HGBEC culture supernatant in a time- and concentration-dependent. The damage to HGBEC in H pylori culture liquid was more significant than that in Hpylori sonicated extracts. CONCLUSION: H pylori induces no obvious damage to HGBEC. (C) 2008 The WIG Press. All rights reserved.