NMR-based Metabonomic Studies on Urine and Serum from Pr(NO3)3-treated Rats

被引：5
作者：
Liao Pei-Qiu [1 ,2 ]
Xue Rong [2 ]
Wu Yi-Jie [2 ]
Pei Feng-Kui [2 ]
Li Xiao-Jing [2 ]
机构：
[1] NE Normal Univ, Dept Chem, Changchun 130024, Peoples R China
[2] Chinese Acad Sci, Changchun Inst Appl Chem, Changchun 130022, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2012年 / 40卷 / 09期
关键词：
Praseodymium nitrate; Biofluids; Nuclear magnetic resonance; Biochemical effects; Pattern recognition; Metabonomics; MAGNETIC-RESONANCE-SPECTROSCOPY; PATTERN-RECOGNITION; H-1-NMR SPECTROSCOPY; TREATED RATS; TOXICITY; CLASSIFICATION; CADMIUM; MODEL;
D O I：
10.3724/SP.J.1096.2012.11185
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The acute biochemical effects of Pr(NO3)(3) were studied by NMR-based metabonomic approach on rats urine and serum. Male Wistar rats were administrated intraperitoneally with Pr(NO3)(3) at doses of 2, 10 and 50 mg/kg body weight. Urine was collected over a 168 h period after dosing and serum was collected at 48 h, 96 h and 168 h period. H-1 NMR spectra of biofluids (urine and serum) were analyzed by principal components analysis. The results revealed that Pr(NO3)(3) caused acute damages on rats liver and kidney, mainly in the liver, and showed a significant dose-dependent relationship. The liver and kidney injury induced by Pr(NO3)(3) was characterized by the variations of many endogenous metabolites, such as citrate, succinate, a-ketoglutarate, creatinine, N-trimethyl dioxide, amino acids, lactate, taurine and glucose.
引用
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页码：1421 / 1428
页数：8
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