Crystal structure of a group II intron in the pre-catalytic state

被引：37

作者：

Chan, Russell T. ^{[1
]}

Robart, Aaron R. ^{[1
]}

Rajashankar, Kanagalaghatta R. ^{[2
]}

Pyle, Anna Marie ^{[3
,4
]}

Toor, Navtej ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, San Diego, CA 92103 USA

[2] Argonne Natl Lab, NE Collaborat Access Team NE CAT, Adv Photon Source, Argonne, IL 60439 USA

[3] Yale Univ, Dept Mol Cellular & Dev Biol, New Haven, CT USA

[4] Howard Hughes Med Inst, Chevy Chase, MD USA

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2012年 / 19卷 / 05期

基金：

美国国家卫生研究院;

关键词：

SELF-SPLICING INTRONS; EXON-BINDING; SITE; RNA;

D O I：

10.1038/nsmb.2270

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Group II introns are self-splicing catalytic RNAs that are thought to be ancestral to the spliceosome. Here we report the 3.65-angstrom crystal structure of the group II intron from Oceanobacillus iheyensis in the pre-catalytic state. The structure reveals the conformation of the 5' splice site in the catalytic core and represents the first structure of an intron prior to the first step of splicing.

引用

页码：555 / 557

页数：3

共 18 条

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