Injectable hydrogels based on MPEG-PCL-RGD and BMSCs for bone tissue engineering

被引：42
|
作者：
Kim, Hyun Joo [1 ,2 ]
You, Su Jung [2 ]
Yang, Dae Hyeok [2 ]
Eun, Jin [3 ]
Park, Hae Kwan [3 ]
Kim, Moon Suk [4 ]
Chun, Heung Jae [1 ,2 ,5 ]
机构：
[1] Catholic Univ Korea, Dept Biomed & Hlth Sci, Seoul 06591, South Korea
[2] Catholic Univ Korea, Inst Cell & Tissue Engn, Seoul 06591, South Korea
[3] Catholic Univ Korea, Coll Med, Eunpyeong St Marys Hosp, Dept Neurosurg, Seoul 03312, South Korea
[4] Ajou Univ, Dept Mol Sci & Technol, Suwon 16499, South Korea
[5] Catholic Univ Korea, Coll Med, Dept Med Life Sci, Seoul 06591, South Korea
来源：
BIOMATERIALS SCIENCE | 2020年 / 8卷 / 15期
基金：
新加坡国家研究基金会;
关键词：
COMPOSITE HYDROGELS; IN-VITRO; BIOMATERIALS; COPOLYMER;
D O I：
10.1039/d0bm00588f
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The aim of this study was to investigate the osteogenic potential of bone marrow-derived mesenchymal stem cells (BMSCs) seeded on novel thermosensitivein situforming hydrogel systems comprising methoxy polyethylene glycol-polycaprolactone (MP) and RGD-conjugated MP (MP-RGD)in vitroandin vivo. Real-time polymerase chain reaction (PCR) together with immunofluorescence staining revealed the strong expression of osteogenic markers (collagen 1 and osteocalcin) of BMSCs in MP/MP-RGD samples compared to MP samples. PCR array testing also showed the upregulation of the interconnected signaling networks regulating cell proliferation and differentiation, which was further verified through the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Histological findings and computed tomographic analysis demonstrated that the MP/MP-RGD hydrogel dramatically promoted new bone formation in a rabbit calvarial defect model. In conclusion, this hydrogel appears to elicit cellular behaviors desired for bone tissue regeneration.
引用
收藏
页码：4334 / 4345
页数：12
相关论文
共 50 条
  • [21] Development of silk fibroin/nanohydroxyapatite composite hydrogels for bone tissue engineering
    Ribeiro, Marta
    de Moraes, Mariana A.
    Beppu, Marisa M.
    Garcia, Monica P.
    Fernandes, Maria H.
    Monteiro, Fernando J.
    Ferraz, Maria P.
    EUROPEAN POLYMER JOURNAL, 2015, 67 : 66 - 77
  • [22] Tissue engineering based on electrochemical desorption of an RGD-containing oligopeptide
    Mochizuki, Naoto
    Kakegawa, Takahiro
    Osaki, Tatsuya
    Sadr, Nasser
    Kachouie, Nezamoddin N.
    Suzuki, Hiroaki
    Fukuda, Junji
    JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 2013, 7 (03) : 236 - 243
  • [23] Synthesis and photopolymerisation of maleic polyvinyl alcohol based hydrogels for bone tissue engineering
    Killion, John A.
    Geever, Luke M.
    Cloonan, Martin
    Grehan, Laura
    Waldron, Cathal
    Quinn, Keith
    Lyons, John
    Devine, Declan M.
    Higginbotham, Clement L.
    JOURNAL OF POLYMER RESEARCH, 2014, 21 (09)
  • [24] The Application of Hyaluronic Acid-Based Hydrogels in Bone and Cartilage Tissue Engineering
    Li, Hongru
    Qi, Zhiping
    Zheng, Shuang
    Chang, Yuxin
    Kong, Weijian
    Fu, Chuan
    Yu, Ziyuan
    Yang, Xiaoyu
    Pan, Su
    ADVANCES IN MATERIALS SCIENCE AND ENGINEERING, 2019, 2019
  • [25] The Application of Hydrogels Based on Natural Polymers for Tissue Engineering
    Taghipour, Yasamin Davatgaran
    Hokmabad, Vahideh Raeisdasteh
    Del Bakhshayesh, Azizeh Rahmani
    Asadi, Nahideh
    Salehi, Roya
    Nasrabadi, Hamid Tayefi
    CURRENT MEDICINAL CHEMISTRY, 2020, 27 (16) : 2658 - 2680
  • [26] Nanoclay-Composite Hydrogels for Bone Tissue Engineering
    Hwang, Hee Sook
    Lee, Chung-Sung
    GELS, 2024, 10 (08)
  • [27] Chitosan/POSS Hybrid Hydrogels for Bone Tissue Engineering
    Celesti, Consuelo
    Iannazzo, Daniela
    Espro, Claudia
    Visco, Annamaria
    Legnani, Laura
    Veltri, Lucia
    Visalli, Giuseppa
    Di Pietro, Angela
    Bottino, Paola
    Chiacchio, Maria Assunta
    MATERIALS, 2022, 15 (22)
  • [28] An in vitro study of two GAG-like marine polysaccharides incorporated into injectable hydrogels for bone and cartilage tissue engineering
    Rederstorff, E.
    Weiss, P.
    Sourice, S.
    Pilet, P.
    Xie, F.
    Sinquin, C.
    Colliec-Jouault, S.
    Guicheux, J.
    Laib, S.
    ACTA BIOMATERIALIA, 2011, 7 (05) : 2119 - 2130
  • [29] Rheological Properties of Injectable Hyaluronic Acid Hydrogels for Soft Tissue Engineering Applications
    Kaya, Gizem
    Oytun, Faruk
    BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY, 2021, 11 (01): : 8424 - 8430
  • [30] Thermosensitive Poly(N-vinylcaprolactam) Injectable Hydrogels for Cartilage Tissue Engineering
    Sala, Renata L.
    Kwon, Mi Y.
    Kim, Minwook
    Gullbrand, Sarah E.
    Henning, Elizabeth A.
    Mauck, Robert L.
    Camargo, Emerson R.
    Burdick, Jason A.
    TISSUE ENGINEERING PART A, 2017, 23 (17-18) : 935 - 945
12345