Injectable hydrogels based on MPEG-PCL-RGD and BMSCs for bone tissue engineering

被引：42
|
作者：
Kim, Hyun Joo [1 ,2 ]
You, Su Jung [2 ]
Yang, Dae Hyeok [2 ]
Eun, Jin [3 ]
Park, Hae Kwan [3 ]
Kim, Moon Suk [4 ]
Chun, Heung Jae [1 ,2 ,5 ]
机构：
[1] Catholic Univ Korea, Dept Biomed & Hlth Sci, Seoul 06591, South Korea
[2] Catholic Univ Korea, Inst Cell & Tissue Engn, Seoul 06591, South Korea
[3] Catholic Univ Korea, Coll Med, Eunpyeong St Marys Hosp, Dept Neurosurg, Seoul 03312, South Korea
[4] Ajou Univ, Dept Mol Sci & Technol, Suwon 16499, South Korea
[5] Catholic Univ Korea, Coll Med, Dept Med Life Sci, Seoul 06591, South Korea
来源：
BIOMATERIALS SCIENCE | 2020年 / 8卷 / 15期
基金：
新加坡国家研究基金会;
关键词：
COMPOSITE HYDROGELS; IN-VITRO; BIOMATERIALS; COPOLYMER;
D O I：
10.1039/d0bm00588f
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
The aim of this study was to investigate the osteogenic potential of bone marrow-derived mesenchymal stem cells (BMSCs) seeded on novel thermosensitivein situforming hydrogel systems comprising methoxy polyethylene glycol-polycaprolactone (MP) and RGD-conjugated MP (MP-RGD)in vitroandin vivo. Real-time polymerase chain reaction (PCR) together with immunofluorescence staining revealed the strong expression of osteogenic markers (collagen 1 and osteocalcin) of BMSCs in MP/MP-RGD samples compared to MP samples. PCR array testing also showed the upregulation of the interconnected signaling networks regulating cell proliferation and differentiation, which was further verified through the Kyoto Encyclopedia of Genes and Genomes pathway analysis. Histological findings and computed tomographic analysis demonstrated that the MP/MP-RGD hydrogel dramatically promoted new bone formation in a rabbit calvarial defect model. In conclusion, this hydrogel appears to elicit cellular behaviors desired for bone tissue regeneration.
引用
收藏
页码：4334 / 4345
页数：12
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