Metabolic engineering of bacterial strains using CRISPR/Cas9 systems for biosynthesis of value-added products

被引：26

作者：

Fokum, Ernest ^{[1
]}

Zabed, Hossain M. ^{[1
]}

Guo, Qi ^{[1
]}

Yun, Junhua ^{[1
]}

Yang, Miaomiao ^{[1
]}

Pang, Hao ^{[2
]}

An, Yingfeng ^{[3
]}

Li, Wen ^{[1
]}

Qi, Xianghui ^{[1
,2
]}

机构：

[1] Jiangsu Univ, Sch Food Sci & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China

[2] Guangxi Acad Sci, Guangxi Key Lab Biorefinery, Nanning 530007, Peoples R China

[3] Shenyang Agr Univ, Coll Biosci & Biotechnol, Shenyang 110161, Liaoning, Peoples R China

来源：

FOOD BIOSCIENCE | 2019年 / 28卷

基金：

中国国家自然科学基金; 中国博士后科学基金;

关键词：

CRISPR/Cas9; Value-added ingredients; Bacterial genome editing; Strain improvement; Microbial biosynthesis; SEQUENCE-SPECIFIC CONTROL; ESCHERICHIA-COLI; GENE-EXPRESSION; INTERFERENCE CRISPRI; BACILLUS-SUBTILIS; GENOME; REPRESSION; BIOTRANSFORMATION; 1,3-PROPANEDIOL; DEHYDROGENASE;

D O I：

10.1016/j.fbio.2019.01.003

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Metabolically engineered bacterial strains are fast becoming the center of attraction in the biosynthesis of various bio-based ingredients. Currently, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) with its associated RNA guided endonuclease (Cas9), is considered as the most promising tool that has brought a breakthrough in the genome editing approach. Although this versatile system originates from the immune systems of bacteria, its application in bacterial genome editing as an approach of metabolic engineering is still not so extensive. This review paper discusses the challenges, opportunities and application of CRISPR/Cas system in bacterial genome editing for biosynthesis of various value-added ingredients. The content of this paper mainly emphasizes Type II CRISPR/Cas9 system used for knock-in and knock-out of various target genes in the bacterial strains as a pre-requisite for increasing the production of bio-based materials.

引用

页码：125 / 132

页数：8

共 41 条

[41] Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems
Kawabe, Yoshinori
Komatsu, Shinya
Komatsu, Shodai
Murakami, Mai
Ito, Akira
Sakuma, Tetsushi
Nakamura, Takahiro
Yamamoto, Takashi
Kamihira, Masamichi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2018, 125 (05) : 599 - 605

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