Polyethyleneimine modified fluorescent carbon dots and their application in cell labeling

被引：132
作者：
Han, Baofu [1 ]
Wang, Wenxing [2 ]
Wu, Hongyan [1 ]
Fang, Fang [1 ]
Wang, Naizhi [1 ]
Zhang, Xiujuan [1 ]
Xu, Shukun [1 ]
机构：
[1] Northeastern Univ, Dept Chem, Shenyang 110819, Peoples R China
[2] Northeastern Univ, Inst Biol Technol, Shenyang 110819, Peoples R China
来源：
COLLOIDS AND SURFACES B-BIOINTERFACES | 2012年 / 100卷
基金：
中国博士后科学基金; 美国国家科学基金会;
关键词：
Fluorescent carbon dots; Solvothermal; Polyethyleneimine; Cell labeling; Cytotoxicity; NANOPARTICLES; NANOCRYSTALS;
D O I：
10.1016/j.colsurfb.2012.05.016
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Fluorescent carbon dots (CDs) were solvothermaly synthesized in water-glycol medium by using glucose as carbon source and then modified with polyethyleneimine (PEI) for the first time to improve fluorescence quality. The as-prepared CDs were monodispersed sphere particles with a diameter of about 7.5 nm, emitting strong fluorescence which is excitation wavelength-dependent, with a quantum yield of 3.5%. After PEI modification, there was a 300-fold enhancement in fluorescence intensity and also a red shift in emission wavelength. The as-prepared CDs were then conjugated with CEA8 antibody to label and image HeLa cells in vitro. We also tested the cytotoxicity of these CDs using HeLa cells. No apparent cytotoxicity was observed, demonstrating much better biosafety property compared with CdTe quantum dots. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码：209 / 214
页数：6
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