A combination of thidiazuron and benzyladenine promotes multiple shoot production from cotyledonary node explants of faba bean (Vicia faba L.)

被引：53

作者：

Khalafalla, MM ^{[1
]}

Hattori, K ^{[1
]}

机构：

[1] Nagoya Univ, Sch Agr Sci, Lab Plant Genet & Breeding, Chikusa Ku, Nagoya, Aichi 4648601, Japan

来源：

PLANT GROWTH REGULATION | 1999年 / 27卷 / 03期

关键词：

cotyledonary node; faba bean; multiple shoot formation; Vicia faba;

D O I：

10.1023/A:1006106328085

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A procedure for multiple shoot formation from cotyledonary node explants of faba bean (Vicia faba L.cv.S.Ghdar) cultured on MS medium containing benzyladenine (BA) and thidiazuron (TDZ) was developed. Explants on medium with TDZ in combination with BA produced a higher number of shoots than with either cytokinin alone. The highest number of shoots was obtained when explants from 7-day-old seedlings were cultured on MS medium supplemented with TDZ and BA (2 mgl(-1) each) for 31 days before transfer to hormone-free MS medium for elongation. Shoots produced in vitro were rooted on half-strength agar-solidified MS basal medium or with 0.25 or 0.5 mgl(-1) naphthalenacetic acid (NAA) prior to transfer to green house conditions. This procedure was found to be applicable to seven other cultivars of faba bean from widely diverse provenances. Thus, it can be advantageously applied to the production of transgenic faba bean plants.

引用

页码：145 / 148

页数：4

共 13 条

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