Detection of circulating tumor cells in peripheral blood from patients with gastric cancer using piRNAs as markers

被引：160

作者：

Cui, Long ^{[1
]}

Lou, Yanru ^{[1
,2
,3
]}

Zhang, Xinjun ^{[1
,4
]}

Zhou, Hui ^{[1
]}

Deng, Hongxia ^{[1
]}

Song, Haojun ^{[1
]}

Yu, Xiuchong ^{[1
]}

Xiao, Bingxiu ^{[1
]}

Wang, Weihua ^{[1
,4
]}

Guo, Junming ^{[1
]}

机构：

[1] Ningbo Univ, Sch Med, Ningbo 315211, Zhejiang, Peoples R China

[2] Ningbo Univ, Sch Med, Affiliated Hosp, Ningbo 315000, Zhejiang, Peoples R China

[3] Ningbo Univ, Sch Med, Ningbo Hosp 1, Ningbo 315000, Zhejiang, Peoples R China

[4] Ningbo Univ, Sch Med, Affiliated Hosp, Ningbo 315010, Zhejiang, Peoples R China

来源：

CLINICAL BIOCHEMISTRY | 2011年 / 44卷 / 13期

基金：

中国国家自然科学基金;

关键词：

Piwi-interacting RNA; Gastric cancer; Circulating tumor cells; Gene diagnosis; MESSENGER-RNA; STEM-CELL; EXPRESSION; MICROMETASTASES; IDENTIFICATION; BIOGENESIS; CARCINOMA; SURGERY; PROTEIN; PLASMA;

D O I：

10.1016/j.clinbiochem.2011.06.004

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Objective: To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. Design and methods: Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in the peripheral blood of 93 patients with gastric cancer and 32 healthy volunteers. Receiver operating characteristic (ROC) curves were constructed to evaluate the diagnostic values. Results: The peripheral blood levels of piR-651 and piR-823 in the patients with gastric cancer were significantly lower than those from controls (P<0.001). The piR-651 level in gastric adenocarcinoma was higher than that in gastric signet ring cell carcinoma (P=0.003). The piR-823 level was positively associated with tumor-node-metastasis stage (P=0.027) and distant metastasis (P=0.026). The areas under the ROC curve were 0.841, 0.812 and 0.860 for piR-651, piR-823 and the combination, respectively. Conclusions: piRNAs may be valuable biomarkers for detecting circulating gastric cancer cells. (C) 2011 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

引用

页码：1050 / 1057

页数：8

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