Multiple targeting motifs direct NRAMP1 into lysosomes

被引：5
|
作者：
Cheng, Xiang [1 ]
Wang, Huayan [1 ]
机构：
[1] NW A&F Univ, Coll Vet Med, Dept Anim Biotechnol, Yangling 712100, Shaanxi, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 419卷 / 03期
关键词：
NRAMP1; Targeting motifs; Lysosome; Trafficking; NATURAL-RESISTANCE; INTRACELLULAR INFECTIONS; ENDOPLASMIC-RETICULUM; SORTING SIGNALS; IN-VITRO; MEMBRANE; PROTEIN; TRAFFICKING; GENE; IDENTIFICATION;
D O I：
10.1016/j.bbrc.2012.02.078
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Natural resistance-associated macrophage protein 1 (NRAMP1) containing 548 amino acids (AA) and 12 transmembrane domains (TMDs) is localized in membranes of lysosomes. Our study aimed to investigate the targeting motifs of NRAMP1 by expressing GFP-tagged full-length and truncated NRAMP1 proteins and overlapping with the lysosomal marker Lamp1-RFP in Chinese hamster ovary (CHO) cells. The NH2-terminal amino acids 73-140 region including TMD2 was essential for NRAMP1 lysosomal targeting. The AA.263-334 region containing the tyrosine-based motif (327)YAPI(330) targeted NRAMP1 into lysosomes. Additionally, two internal signal peptides AA.451-483 and AA.489-522 were identified as lysosomal targeting motifs. Taken together, NRAMP1 consists of multiple targeting motifs for trafficking into lysosomes. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码：578 / 583
页数：6
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