Multiple targeting motifs direct NRAMP1 into lysosomes

被引：5

作者：

Cheng, Xiang ^{[1
]}

Wang, Huayan ^{[1
]}

机构：

[1] NW A&F Univ, Coll Vet Med, Dept Anim Biotechnol, Yangling 712100, Shaanxi, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 419卷 / 03期

关键词：

NRAMP1; Targeting motifs; Lysosome; Trafficking; NATURAL-RESISTANCE; INTRACELLULAR INFECTIONS; ENDOPLASMIC-RETICULUM; SORTING SIGNALS; IN-VITRO; MEMBRANE; PROTEIN; TRAFFICKING; GENE; IDENTIFICATION;

D O I：

10.1016/j.bbrc.2012.02.078

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Natural resistance-associated macrophage protein 1 (NRAMP1) containing 548 amino acids (AA) and 12 transmembrane domains (TMDs) is localized in membranes of lysosomes. Our study aimed to investigate the targeting motifs of NRAMP1 by expressing GFP-tagged full-length and truncated NRAMP1 proteins and overlapping with the lysosomal marker Lamp1-RFP in Chinese hamster ovary (CHO) cells. The NH2-terminal amino acids 73-140 region including TMD2 was essential for NRAMP1 lysosomal targeting. The AA.263-334 region containing the tyrosine-based motif (327)YAPI(330) targeted NRAMP1 into lysosomes. Additionally, two internal signal peptides AA.451-483 and AA.489-522 were identified as lysosomal targeting motifs. Taken together, NRAMP1 consists of multiple targeting motifs for trafficking into lysosomes. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：578 / 583

页数：6

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