Multiple targeting motifs direct NRAMP1 into lysosomes

被引：5

作者：

Cheng, Xiang ^{[1
]}

Wang, Huayan ^{[1
]}

机构：

[1] NW A&F Univ, Coll Vet Med, Dept Anim Biotechnol, Yangling 712100, Shaanxi, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 419卷 / 03期

关键词：

NRAMP1; Targeting motifs; Lysosome; Trafficking; NATURAL-RESISTANCE; INTRACELLULAR INFECTIONS; ENDOPLASMIC-RETICULUM; SORTING SIGNALS; IN-VITRO; MEMBRANE; PROTEIN; TRAFFICKING; GENE; IDENTIFICATION;

D O I：

10.1016/j.bbrc.2012.02.078

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Natural resistance-associated macrophage protein 1 (NRAMP1) containing 548 amino acids (AA) and 12 transmembrane domains (TMDs) is localized in membranes of lysosomes. Our study aimed to investigate the targeting motifs of NRAMP1 by expressing GFP-tagged full-length and truncated NRAMP1 proteins and overlapping with the lysosomal marker Lamp1-RFP in Chinese hamster ovary (CHO) cells. The NH2-terminal amino acids 73-140 region including TMD2 was essential for NRAMP1 lysosomal targeting. The AA.263-334 region containing the tyrosine-based motif (327)YAPI(330) targeted NRAMP1 into lysosomes. Additionally, two internal signal peptides AA.451-483 and AA.489-522 were identified as lysosomal targeting motifs. Taken together, NRAMP1 consists of multiple targeting motifs for trafficking into lysosomes. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：578 / 583

页数：6

共 37 条

[1] GENETIC-REGULATION OF LEISHMANIAL AND MYCOBACTERIAL INFECTIONS - THE LSH/ITY/BCG GENE STORY CONTINUES [J].

BLACKWELL, JM ;

BARTON, CH ;

WHITE, JK ;

ROACH, TIA ;

SHAW, MA ;

WHITEHEAD, SH ;

MOCK, BA ;

SEARLE, S ;

WILLIAMS, H ;

BAKER, AM .

IMMUNOLOGY LETTERS, 1994, 43 (1-2) :99-107

[2] Signals for sorting of transmembrane proteins to endosomes and lysosomes [J].

Bonifacino, JS ;

Traub, LM .

ANNUAL REVIEW OF BIOCHEMISTRY, 2003, 72 :395-447

[3] Sorting of lysosomal proteins [J].

Braulke, Thomas ;

Bonifacino, Juan S. .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2009, 1793 (04) :605-614

[4] Multiple cytosolic and transmembrane determinants are required for the trafficking of SCAMP1 via an ER-Golgi-TGN-PM pathway [J].

Cai, Yi ;

Jia, Tianran ;

Lam, Sheung Kwan ;

Ding, Yu ;

Gao, Caiji ;

San, Melody Wan Yan ;

Pimpl, Peter ;

Jiang, Liwen .

PLANT JOURNAL, 2011, 65 (06) :882-896

[5] Expression and subcellular localization of NRAMP1 in human neutrophil granules [J].

Canonne-Hergaux, F ;

Calafat, J ;

Richer, E ;

Cellier, M ;

Grinstein, S ;

Borregaard, N ;

Gros, P .

BLOOD, 2002, 100 (01) :268-275

[6]

Carmel S.B., 2007, INFECT IMMUN, V75, P5059

[7] Resistance to intracellular infections: Comparative genomic analysis of Nramp [J].

Cellier, M ;

Belouchi, A ;

Gros, P .

TRENDS IN GENETICS, 1996, 12 (06) :201-204

[8] Polyphyletic origins of bacterial Nramp transporters [J].

Cellier, MFM ;

Bergevin, I ;

Boyer, E ;

Richer, E .

TRENDS IN GENETICS, 2001, 17 (07) :365-370

[9] The targeting of cystinosin to the lysosomal membrane requires a tyrosine-based signal and a novel sorting motif [J].

Cherqui, S ;

Kalatzis, V ;

Trugnan, G ;

Antignac, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (16) :13314-13321

[10] Regulation and function of the CD3 gamma DxxxLL motif: A binding site for adaptor protein-1 and adaptor protein-2 in vitro [J].

Dietrich, J ;

Kastrup, J ;

Nielsen, BL ;

Odum, N ;

Geisler, C .

JOURNAL OF CELL BIOLOGY, 1997, 138 (02) :271-281

← 1 2 3 4 →