Human NK Cell Subset Functions Are Differentially Affected by Adipokines

被引:26
|
作者
Huebner, Lena [1 ,2 ]
Engeli, Stefan [3 ]
Wrann, Christiane D. [4 ]
Goudeva, Lilia [5 ]
Laue, Tobias [1 ]
Kielstein, Heike [6 ]
机构
[1] Hannover Med Sch, Inst Funct & Appl Anat, Hannover, Germany
[2] Univ Med Berlin, Charite, Dept Gen Visceral Vasc & Thorac Surg, Berlin, Germany
[3] Hannover Med Sch, Inst Clin Pharmacol, Hannover, Germany
[4] Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Endocrinol, Boston, MA 02215 USA
[5] Hannover Med Sch, Inst Transfus Med, Hannover, Germany
[6] Univ Halle Wittenberg, Dept Anat & Cell Biol, Fac Med, D-06108 Halle, Saale, Germany
来源
PLOS ONE | 2013年 / 8卷 / 09期
关键词
NATURAL-KILLER-CELLS; ADIPOSE-TISSUE; IFN-GAMMA; OBESITY; LEPTIN; INFLAMMATION; ADIPONECTIN; DYSFUNCTION; CYTOKINES; IDENTIFICATION;
D O I
10.1371/journal.pone.0075703
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Obesity is a risk factor for various types of infectious diseases and cancer. The increase in adipose tissue causes alterations in both adipogenesis and the production of adipocyte-secreted proteins (adipokines). Since natural killer (NK) cells are the host's primary defense against virus-infected and tumor cells, we investigated how adipocyte-conditioned medium (ACM) affects functions of two distinct human NK cell subsets. Methods: Isolated human peripheral blood mononuclear cells (PBMCs) were cultured with various concentrations of human and murine ACM harvested on two different days during adipogenesis and analyzed by fluorescent-activated cell sorting (FACS). Results: FACS analyses showed that the expression of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), granzyme A (GzmA) and interferon (IFN)-gamma in NK cells was regulated in a subset-specific manner. ACM treatment altered IFN-gamma expression in CD56(dim) NK cells. The production of GzmA in CD56(bright) NK cells was differentially affected by the distinct adipokine compositions harvested at different states of adipogenesis. Comparison of the treatment with either human or murine ACM revealed that adipokine-induced effects on NK cell expression of the leptin receptor (Ob-R), TRAIL and IFN-gamma were species-specific. Conclusion: Considering the growing prevalence of obesity and the various disorders related to it, the present study provides further insights into the roles human NK cell subsets play in the obesity-associated state of chronic low-grade inflammation.
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页数:11
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