Supercoil-Accelerated DNA Threading Intercalation

被引：8

作者：

Nordell, Par ^{[1
]}

Jansson, Erik T. ^{[1
]}

Lincoln, Per ^{[1
]}

机构：

[1] Chalmers, Dept Chem & Biol Engn, SE-41296 Gothenburg, Sweden

来源：

BIOCHEMISTRY | 2009年 / 48卷 / 07期

关键词：

RNA-POLYMERASE; BINDING; TRANSCRIPTION; COMPLEXES; KINETICS; DISSOCIATION; EQUILIBRIUM; ACTINOMYCIN; INITIATION; PROMOTERS;

D O I：

10.1021/bi802336p

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further saturation is on the other hand kinetically hampered in comparison to the relaxed DNA. We also observe that threading kinetics correlates with the inhibition of luciferase expression from the plasmid construct. The results show how the target torsional strain can function as a control of DNA threading kinetics and gene expression efficiency.

引用

页码：1442 / 1444

页数：3

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