Eriochrome Black T-Eu3+ Complex as a Ratiometric Colorimetric and Fluorescent Probe for the Detection of Dipicolinic Acid, a Biomarker of Bacterial Spores

被引：107

作者：

Yilmaz, M. Deniz ^{[1
,2
]}

Oktem, Huseyin Avni ^{[3
,4
]}

机构：

[1] Konya Food & Agr Univ, Dept Bioengn, Fac Engn & Architecture, TR-42080 Konya, Turkey

[2] Konya Food & Agr Univ, Res & Dev Ctr Diagnost Kits KIT ARGEM, TR-42080 Konya, Turkey

[3] Middle East Tech Univ, Dept Biol Sci, TR-06800 Ankara, Turkey

[4] Nanobiz R&D Ltd, Gallium Bld 18,METU Sci Pk, Ankara, Turkey

来源：

ANALYTICAL CHEMISTRY | 2018年 / 90卷 / 06期

关键词：

CIRCULARLY-POLARIZED LUMINESCENCE; ANTHRAX BIOMARKER; INHALATIONAL ANTHRAX; RAPID DETECTION; BIOTERRORISM; SENSOR; ASSAY;

D O I：

10.1021/acs.analchem.8b00576

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel ratiometric colorimetric and fluorescent dual probe based on Eriochrome Black T (EBT) Eu3+ complex was designed to detect dipicolinic acid (DPA), a major constituent of bacterial spores, with high sensitivity and selectivity. UV vis titration experiments demonstrated that EBT and Eu3+ ions formed a 1:1 coordination pair in water. In the presence of Eu3+ ions, the blue solution of EBT changed to magenta, however, upon the addition of DPA, the magenta color changed to blue immediately and characteristic fluorescence emission from DPA-Eu3+ complex was observed. In addition, the sensitivity of the system was further evaluated on Geobacillus stearothermophilus spores and as low as 2.5 X 10(5) spores were detected.

引用

收藏

页码：4221 / 4225

页数：5

相关论文

共 34 条

[1] Europium-Based Fluorescence Nanoparticle Sensor for Rapid and Ultrasensitive Detection of an Anthrax Biomarker [J].

Ai, Kelong ;

Zhang, Baohua ;

Lu, Lehui .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (02) :304-308

[2] Biological indicators for steam sterilization:: characterization of a rapid biological indicator utilizing Bacillus stearothermophilus spore-associated alpha-glucosidase enzyme [J].

Albert, H ;

Davies, DJG ;

Woodson, LP ;

Soper, CJ .

JOURNAL OF APPLIED MICROBIOLOGY, 1998, 85 (05) :865-874

[3] STRUCTURE AND MORPHOGENESIS OF BACTERIAL SPORE COAT [J].

ARONSON, AI ;

FITZJAMES, P .

BACTERIOLOGICAL REVIEWS, 1976, 40 (02) :360-402

[4] Bacterial spore detection by [Tb3+(macrocycle)(dipicolinate)] luminescence [J].

Cable, Morgan L. ;

Kirby, James P. ;

Sorasaenee, Karn ;

Gray, Harry B. ;

Ponce, Adrian .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (06) :1474-1475

[5] Detection of Bacterial Spores with Lanthanide-Macrocycle Binary Complexes [J].

Cable, Morgan L. ;

Kirby, James P. ;

Levine, Dana J. ;

Manary, Micah J. ;

Gray, Harry B. ;

Ponce, Adrian .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (27) :9562-9570

[6] A ratiometric fluorescent nanoprobe based on terbium functionalized carbon dots for highly sensitive detection of an anthrax biomarker [J].

Chen, Hao ;

Xie, Yujie ;

Kirillov, Alexander M. ;

Liu, Liangliang ;

Yu, Minghui ;

Liu, Weisheng ;

Tang, Yu .

CHEMICAL COMMUNICATIONS, 2015, 51 (24) :5036-5039

[7] Dual colorimetric and luminescent assay for dipicolinate, a biomarker of bacterial spores [J].

Clear, Kasey J. ;

Stroud, Sarah ;

Smith, Bradley D. .

ANALYST, 2013, 138 (23) :7079-7082

[8] Fluorescent detection of dipicolinic acid as a biomarker of bacterial spores using lanthanide-chelated gold nanoparticles [J].

Donmez, Mert ;

Yilmaz, M. Deniz ;

Kilbas, Benan .

JOURNAL OF HAZARDOUS MATERIALS, 2017, 324 :593-598

[9] A Supramolecular Sensing Platform for Phosphate Anions and an Anthrax Biomarker in a Microfluidic Device [J].

Eker, Bilge ;

Yilmaz, Mahmut Deniz ;

Schlautmann, Stefan ;

Gardeniers, Johannes G. E. ;

Huskens, Jurriaan .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2011, 12 (11) :7335-7351

[10] Bioterrorism - This time it was real: Knowledge of anthrax put to the test [J].

Enserink, M .

SCIENCE, 2001, 294 (5542) :490-491

← 1 2 3 4 →