Mononuclear cell collection for extracorporeal photochemotherapy: a study comparing an automatic and a semiautomatic apheresis device

被引:20
作者
Del Fante, Claudia
Scudeller, Luigia
Viarengo, Gianluca
Cervio, Marila
Perotti, Cesare
机构
[1] Fdn IRCCS Policlin San Matteo, Immunohaematol & Transfus Serv, Apheresis & Cell Therapy Unit, Pavia, Italy
[2] Fdn IRCCS Policlin San Matteo, Sci Direct, Pavia, Italy
关键词
VERSUS-HOST-DISEASE; TRANSPLANTATION; PHOTOPHERESIS; SURVIVAL; LYMPHOMA;
D O I
10.1111/trf.12065
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Extracorporeal photochemotherapy (ECP) is an effective cell therapy employed in several diseases, including graft-versus-host disease (GVHD) and organ rejection after transplantation. When ECP is performed using the off-line technique, mononuclear cell (MNC) collection by leukapheresis is necessary for further manipulation. Semiautomated apheresis systems require experienced personnel to obtain a good MNC collection; an automated device, able to efficiently collect MNCs with high purity, is desirable. We compared the semiautomated COBE Spectra MNC and the new automated Spectra Optia v.5.0 MNC (Terumo BCT) devices in terms of efficacy and safety. Study Design and Methods: Adult patients with GVHD or bronchiolitis obliterans syndrome (BOS) after lung transplant undergoing ECP at our center were alternatively assigned, within the same ECP cycle (composed by two procedures each), to MNC collection with either device. Patients' characteristics, procedure, and product-related variables were compared. Results: Thirty-nine patients (24 with GVHD and 15 with BOS) underwent a total of 126 ECP procedures, with good compliance to both devices. Product volume and platelet (PLT) and red blood cell contamination were significantly lower with the Spectra Optia. MNC collection efficiency (CE), purity, and PLT loss were similar between the two devices, while white blood cells CE was in favor of the COBE Spectra. Conclusion: The Spectra Optia device proved to be a good option for MNC collection in the difficult ECP setting, since it ensures high-quality MNC collection, while at the same time saving personnel's time, guaranteeing optimal monitoring and care to this frail patient population.
引用
收藏
页码:2027 / 2033
页数:7
相关论文
共 18 条
[1]   Extracorporeal Photopheresis After Lung Transplantation: A 10-Year Single-Center Experience [J].
Benden, Christian ;
Speich, Rudolf ;
Hofbauer, Guenther E. ;
Irani, Sarosh ;
Eich-Wanger, Christine ;
Russi, Erich W. ;
Weder, Walter ;
Boehler, Annette .
TRANSPLANTATION, 2008, 86 (11) :1625-1627
[2]   Extracorporeal photopheresis induces apoptosis in the lymphocytes of cutaneous T-cell lymphoma and graft-versus-host disease patients [J].
Bladon, J ;
Taylor, PC .
BRITISH JOURNAL OF HAEMATOLOGY, 1999, 107 (04) :707-711
[3]   Mobilized allogeneic peripheral stem/progenitor cell apheresis with Spectra Optia v.5.0, a novel, automatic interface-controlled apheresis system: results from the first feasibility trial [J].
Brauninger, S. ;
Bialleck, H. ;
Thorausch, K. ;
Seifried, E. ;
Bonig, H. .
VOX SANGUINIS, 2011, 101 (03) :237-246
[4]  
Brauninger S, 2011, TRANSFUSION, V52, P1137
[5]   The rise of cellular therapy [J].
Choate, Jacquelyn ;
Snyder, Edward L. .
TRANSFUSION AND APHERESIS SCIENCE, 2011, 45 (01) :91-97
[6]   Response and survival of patients with chronic graft-versus-host disease treated by extracorporeal photochemotherapy: a retrospective study according to classical and National Institutes of Health classifications [J].
Del Fante, Claudia ;
Scudeller, Luigia ;
Viarengo, Gianluca ;
Bernasconi, Paolo ;
Perotti, Cesare .
TRANSFUSION, 2012, 52 (09) :2007-2015
[7]   TREATMENT OF CUTANEOUS T-CELL LYMPHOMA BY EXTRACORPOREAL PHOTOCHEMOTHERAPY - PRELIMINARY-RESULTS [J].
EDELSON, R ;
BERGER, C ;
GASPARRO, F ;
JEGASOTHY, B ;
HEALD, P ;
WINTROUB, B ;
VONDERHEID, E ;
KNOBLER, R ;
WOLFF, K ;
PLEWIG, G ;
MCKIERNAN, G ;
CHRISTIANSEN, I ;
OSTER, M ;
HONIGSMANN, H ;
WILFORD, H ;
KOKOSCHKA, E ;
REHLE, T ;
PEREZ, M ;
STINGL, G ;
LAROCHE, L .
NEW ENGLAND JOURNAL OF MEDICINE, 1987, 316 (06) :297-303
[8]   How we mobilize haemopoietic stem cells [J].
Herbert, K. E. ;
Levesque, J. -P. ;
Mills, A. K. ;
Gottlieb, D. J. ;
Cooney, J. ;
Szer, J. ;
Rasko, J. ;
To, L. B. .
INTERNAL MEDICINE JOURNAL, 2011, 41 (08) :588-594
[9]   Inverse relationship between patient peripheral blood CD34+ cell counts and collection efficiency for CD34+ cells in two automated leukapheresis systems [J].
Heuft, HG ;
Dubiel, M ;
Rick, O ;
Kingreen, D ;
Serke, S ;
Schwella, N .
TRANSFUSION, 2001, 41 (08) :1008-1013
[10]   Extracorporeal photopheresis: From solid organs to face transplantation [J].
Hivelin, Mikael ;
Siemionow, Maria ;
Grimbert, Philippe ;
Lantieri, Laurent .
TRANSPLANT IMMUNOLOGY, 2009, 21 (03) :117-128