Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

被引：71
作者：
Johnston, Chris J. C. [1 ]
Robertson, Elaine [1 ]
Harcus, Yvonne [1 ]
Grainger, John R. [2 ]
Coakley, Gillian [1 ]
Smyth, Danielle J. [1 ]
McSorley, Henry J. [1 ]
Maizels, Rick [1 ]
机构：
[1] Univ Edinburgh, Inst Immunol & Infect Res, Edinburgh EH8 9YL, Midlothian, Scotland
[2] Manchester Collaborat Ctr Inflammat Res, Manchester, Lancs, England
来源：
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2015年 / 98期
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
Immunology; Issue; 98; Heligmosomoides polygyrus; Helminth; Life Cycle; Excretory-Secretory Products; Infection; Mouse; ALLERGIC AIRWAY INFLAMMATION; TYPE-2; IMMUNITY; MEDIATED REGULATION; HELMINTH INFECTION; PROTEOMIC ANALYSIS; T-CELLS; RESISTANCE; MODEL; ABSENCE; BAKERI;
D O I：
10.3791/52412
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).
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页数：10
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