Cultivation of Heligmosomoides Polygyrus: An Immunomodulatory Nematode Parasite and its Secreted Products

被引：74

作者：

Johnston, Chris J. C. ^{[1
]}

Robertson, Elaine ^{[1
]}

Harcus, Yvonne ^{[1
]}

Grainger, John R. ^{[2
]}

Coakley, Gillian ^{[1
]}

Smyth, Danielle J. ^{[1
]}

McSorley, Henry J. ^{[1
]}

Maizels, Rick ^{[1
]}

机构：

[1] Univ Edinburgh, Inst Immunol & Infect Res, Edinburgh EH8 9YL, Midlothian, Scotland

[2] Manchester Collaborat Ctr Inflammat Res, Manchester, Lancs, England

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2015年 / 98期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

Immunology; Issue; 98; Heligmosomoides polygyrus; Helminth; Life Cycle; Excretory-Secretory Products; Infection; Mouse; ALLERGIC AIRWAY INFLAMMATION; TYPE-2; IMMUNITY; MEDIATED REGULATION; HELMINTH INFECTION; PROTEOMIC ANALYSIS; T-CELLS; RESISTANCE; MODEL; ABSENCE; BAKERI;

D O I：

10.3791/52412

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).

引用

页数：10

共 47 条

[1] An enteric helminth infection protects against an allergic response to dietary antigen [J].

Bashir, MEH ;

Andersen, P ;

Fuss, IJ ;

Shi, HN ;

Nagler-Anderson, C .

JOURNAL OF IMMUNOLOGY, 2002, 169 (06) :3284-3292

[2] Heligmosomoides bakeri: a model for exploring the biology and genetics of resistance to chronic gastrointestinal nematode infections [J].

Behnke, J. M. ;

Menge, D. M. ;

Noyes, H. .

PARASITOLOGY, 2009, 136 (12) :1565-1580

[3] Genetic variation in resistance to repeated infections with Heligmosomoides polygyrus bakeri, in inbred mouse strains selected for the mouse genome project [J].

Behnke, JM ;

Mugambi, JM ;

Clifford, S ;

Iraqi, FA ;

Baker, RL ;

Gibson, JP ;

Wakelin, D .

PARASITE IMMUNOLOGY, 2006, 28 (03) :85-94

[4] GENETIC-CONTROL OF IMMUNITY TO NEMATOSPIROIDES-DUBIUS - A 9-DAY ANTHELMINTIC ABBREVIATED IMMUNIZING REGIME WHICH SEPARATES WEAK AND STRONG RESPONDER STRAINS OF MICE [J].

BEHNKE, JM ;

ROBINSON, M .

PARASITE IMMUNOLOGY, 1985, 7 (03) :235-253

[5] Toll-like receptor 4 agonists adsorbed to aluminium hydroxide adjuvant attenuate ovalbumin-specific allergic airway disease:: role of MyD88 adaptor molecule and interleukin-12/interferon-γ axis [J].

Bortolatto, J. ;

Borducchi, E. ;

Rodriguez, D. ;

Keller, A. C. ;

Faquim-Mauro, E. ;

Bortoluci, K. R. ;

Mucida, D. ;

Gomes, E. ;

Christ, A. ;

Schnyder-Candrian, S. ;

Schnyder, B. ;

Ryffel, B. ;

Russo, M. .

CLINICAL AND EXPERIMENTAL ALLERGY, 2008, 38 (10) :1668-1679

[6] Heligmosomoides polygyrus: EAE remission is correlated with different systemic cytokine profiles provoked by L4 and adult nematodes [J].

Donskow-Lysoniewska, Katarzyna ;

Krawczak, Katarzyna ;

Doligalska, Maria .

EXPERIMENTAL PARASITOLOGY, 2012, 132 (02) :243-248

[7] The role of OX40 ligand interactions in the development of the Th2 response to the gastrointestinal nematode parasite Heligmosomoides polygyrus [J].

Ekkens, MJ ;

Liu, ZG ;

Liu, Q ;

Whitmire, J ;

Xiao, SY ;

Foster, A ;

Pesce, J ;

VanNoy, J ;

Sharpe, AH ;

Urban, JF ;

Gause, WC .

JOURNAL OF IMMUNOLOGY, 2003, 170 (01) :384-393

[8] Memory Th2 effector cells can develop in the absence of B7-1/B7-2, CD28 interactions, and effector Th cells after priming with an intestinal nematode parasite [J].

Ekkens, MJ ;

Liu, ZG ;

Liu, Q ;

Foster, A ;

Whitmire, J ;

Pesce, J ;

Sharpe, AH ;

Urban, JF ;

Gause, WC .

JOURNAL OF IMMUNOLOGY, 2002, 168 (12) :6344-6351

[9] Innate and adaptive type 2 immune cell responses in genetically controlled resistance to intestinal helminth infection [J].

Filbey, Kara J. ;

Grainger, John R. ;

Smith, Katherine A. ;

Boon, Louis ;

van Rooijen, Nico ;

Harcus, Yvonne ;

Jenkins, Stephen ;

Hewitson, James P. ;

Maizels, Rick M. .

IMMUNOLOGY AND CELL BIOLOGY, 2014, 92 (05) :436-448

[10] IL-21 receptor signaling is integral to the development of Th2 effector responses in vivo [J].

Frohlich, Anja ;

Marsland, Benjamin J. ;

Sonderegger, Ivo ;

Kurrer, Michael ;

Hodge, Martin R. ;

Harris, Nicola L. ;

Kopf, Manfred .

BLOOD, 2007, 109 (05) :2023-2031

← 1 2 3 4 5 →