A rapid loop-mediated isothermal amplification (LAMP) method for detection of the macrolide-streptogramin type B resistance gene msrA in Staphylococcus aureus

被引：12

作者：

Mu, Xiao-Qin ^{[1
,2
]}

Liu, Bin-Bin ^{[1
,2
]}

Hui, Ephraim ^{[1
,2
,3
]}

Huang, William ^{[1
,2
,3
]}

Yao, Li-Chen ^{[1
,2
]}

Duo, Li-Bo ^{[4
]}

Sun, Wen-Ying ^{[4
]}

Li, Gui-Qiu ^{[5
]}

Wang, Fu-Xiang ^{[6
]}

Liu, Shu-Lin ^{[1
,2
,3
,7
]}

机构：

[1] Harbin Med Univ, Systemom Ctr, Coll Pharm, Harbin, Peoples R China

[2] Harbin Med Univ, Genom Res Ctr, State Prov Key Labs Biomed Pharmaceut China, Harbin, Peoples R China

[3] Harbin Med Univ, HMU UCFM Ctr Infect & Genom, Harbin, Peoples R China

[4] Harbin Med Univ, Affiliated Hosp 2, Dept Lab Med, Harbin, Peoples R China

[5] Harbin Med Univ, Affiliated Hosp 1, Dept Lab Diag, Harbin, Peoples R China

[6] Harbin Med Univ, Affiliated Hosp 4, Dept Infect Dis, Harbin, Peoples R China

[7] Univ Calgary, Dept Microbiol Immunol & Infect Dis, Calgary, AB, Canada

来源：

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE | 2016年 / 7卷

基金：

中国国家自然科学基金;

关键词：

Staphylococcus aureus; Diagnostics; LAMP; msrA gene; Antimicrobial resistance; ERYTHROMYCIN RESISTANCE; LINCOSAMIDE; STREPTOCOCCUS; ENTEROCOCCUS; BACTEREMIA; DIAGNOSIS; STRAINS; MSR(A); ASSAY;

D O I：

10.1016/j.jgar.2016.07.006

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Macrolide streptogramin type B resistance (the MSB phenotype) is a multidrug resistance phenotype in Staphylococcus aureus conferred by the resistance gene msrA. However, bacteria having the MSB phenotype are susceptible to lincosamides and 16-membered ring macrolides, which makes profiling resistance genes necessary and urgent for timely and appropriate use of antimicrobials. In this study, the loop-mediated isothermal amplification (LAMP) assay was optimized for prompt detection of the msrA gene. msrA gene sequences were obtained from the National Center for Biotechnology Information (NCBI) database and primers were designed using the LAMP primer designing software PrimerExplorer v.4, which together recognize seven distinct regions of the msrA gene. The specific LAMP primer set designed in this study could amplify the msrA gene within 25 min at an isothermal temperature of 62 degrees C. More importantly, the msrA gene could be detected at a sensitivity as low as 100 pg. Furthermore, this optimized LAMP assay provided swift detection of the msrA gene even directly from human specimens. In conclusion, this assay may have great clinical application potential for detection of the msrA gene. (C) 2016 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.

引用

页码：53 / 58

页数：6

共 30 条

[11] Loop-mediated isothermal amplification: Rapid and sensitive detection of the antibiotic resistance gene ISAba1-blaOXA-51-like in Acinetobacter baumannii [J].

Mu, Xiaoqin ;

Nakano, Ryuichi ;

Nakano, Akiyo ;

Ubagai, Tsuneyuki ;

Kikuchi-Ueda, Takane ;

Tansho-Nagakawa, Shigeru ;

Kikuchi, Hirotoshi ;

Kamoshida, Go ;

Endo, Shiro ;

Yano, Hisakazu ;

Ono, Yasuo .

JOURNAL OF MICROBIOLOGICAL METHODS, 2016, 121 :36-40

[12] Comparison of nucleic acid sequence-based amplification and loop-mediated isothermal amplification for diagnosis of human African trypanosomiasis [J].

Mugasa, Claire M. ;

Katiti, Diana ;

Boobo, Alex ;

Lubega, George W. ;

Schallig, Henk D. F. H. ;

Matovu, Enock .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2014, 78 (02) :144-148

[13]

Natrajsetty HSS, 2015, AM J CASE REP, V16, P236, DOI 10.12659/AJCR.893072

[14] Loop-mediated isothermal amplification of DNA [J].

Notomi, Tsugunori ;

Okayama, Hiroto ;

Masubuchi, Harumi ;

Yonekawa, Toshihiro ;

Watanabe, Keiko ;

Amino, Nobuyuki ;

Hase, Tetsu .

NUCLEIC ACIDS RESEARCH, 2000, 28 (12)

[15] Staphylococcus efflux msr(A) gene characterized in Streptococcus, Enterococcus, Corynebacterium, and Pseudomonas isolates [J].

Ojo, KK ;

Striplin, MJ ;

Ulep, CC ;

Close, NS ;

Zittle, J ;

Luis, H ;

Bernardo, M ;

Leitao, J ;

Roberts, MC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2006, 50 (03) :1089-1091

[16] Development of a loop-mediated isothermal amplification assay for detection of Trichomonas vaginalis [J].

Reyes, John Carlo B. ;

Solon, Juan Antonio A. ;

Rivera, Windell L. .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2014, 79 (03) :337-341

[17] Msr(A) and related macrolide/streptogramin resistance determinants:: incomplete transporters? [J].

Reynolds, E ;

Ross, JI ;

Cove, JH .

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2003, 22 (03) :228-236

[18] INDUCIBLE ERYTHROMYCIN RESISTANCE IN STAPHYLOCOCCI IS ENCODED BY A MEMBER OF THE ATP-BINDING TRANSPORT SUPERGENE FAMILY [J].

ROSS, JI ;

EADY, EA ;

COVE, JH ;

CUNLIFFE, WJ ;

BAUMBERG, S ;

WOOTTON, JC .

MOLECULAR MICROBIOLOGY, 1990, 4 (07) :1207-1214

[19] Minimal functional system required for expression of erythromycin resistance by msrA in Staphylococcus aureus RN4220 [J].

Ross, JI ;

Eady, EA ;

Cove, JH ;

Baumberg, S .

GENE, 1996, 183 (1-2) :143-148

[20]

Saribas Z, 2010, MIKROBIYOL BUL, V44, P177

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