A novel umami electrochemical biosensor based on AuNPs@ZIF-8/Ti3C2 MXene immobilized T1R1-VFT

被引：48

作者：

Liu, Jing ^{[1
]}

Zhang, Ninglong ^{[2
]}

Li, Jiansen ^{[1
]}

Li, Mingyang ^{[2
]}

Wang, Guangxian ^{[1
]}

Wang, Wenli ^{[2
]}

Fan, Yuxia ^{[2
]}

Jiang, Shui ^{[2
]}

Chen, Gaole ^{[2
]}

Zhang, Yin ^{[3
]}

Sun, Xia ^{[1
]}

Liu, Yuan ^{[2
]}

机构：

[1] Shandong Univ Technol, Sch Agr Engn & Food Sci, Zibo 255049, Shandong, Peoples R China

[2] Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Food Sci & Technol, Shanghai 200240, Peoples R China

[3] Chengdu Univ, Key Lab Meat Proc Sichuan, Chengdu 610106, Peoples R China

来源：

FOOD CHEMISTRY | 2022年 / 397卷

基金：

中国国家自然科学基金;

关键词：

Umami electrochemical biosensor; T1R1 Venus flytrap (VFT); AuNPs@ZIF-8/Ti3C2 MXene; Immobilization; BIOELECTRONIC TONGUE; TASTE; NANOPARTICLES; PLATFORM;

D O I：

10.1016/j.foodchem.2022.133838

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The bioelectronic tongues based on taste receptors have been emerging with human-like taste perception. However, the practical applications of the receptor-based biosensors were restricted by their narrow and low dynamic ranges. Here, a novel immobilization strategy based on AuNPs@ZIF-8/Ti3C2 MXene was developed to immobilize the umami ligand binding domain (T1R1-VFT), to fabricate an umami biosensor for umami substances detection. Through the synergic effect of AuNPs@ZIF-8 and Ti3C2 MXene, the capacity to load T1R1-VFT was effectively increased, and the response signal was also amplified by approximately 3 times. The proposed biosensor showed an ultrawide dynamic range of 10(-11)-10(-3) M, and a high upper limit of detection, which was closer to the human taste threshold and suitable for detecting foods rich in umami substances. Additionally, the biosensor was successfully applied to detect real samples and analyze the synergistic effects of binary umami substances.

引用

页数：10

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